Geng Xinze, Zuo Jincheng, Meng Yunhao, Zhuge Yanhui, Zhu Ping, Wu Nan, Bai Xinfu, Ni Guangyan, Hou Yuping
College of Life Sciences Ludong University Yantai China.
School of Resources and Environmental Engineering Ludong University Yantai China.
Ecol Evol. 2023 Oct 9;13(10):e10593. doi: 10.1002/ece3.10593. eCollection 2023 Oct.
The soil fungal community plays an important role in forest ecosystems and is crucially influenced by forest secondary succession. However, the driving factors of fungal community and function during temperate forest succession and their potential impact on succession processes remain poorly understood. In this study, we investigated the dynamics of the soil fungal community in three temperate forest secondary successional stages (shrublands, coniferous forests, and deciduous broad-leaved forests) using high-throughput DNA sequencing coupled with functional prediction via the FUNGuild database. We found that fungal community richness, α-diversity, and evenness decreased significantly during the succession process. Soil available phosphorus and nitrate nitrogen decreased significantly after initial succession occurred, and redundancy analysis showed that both were significant predictors of soil fungal community structure. Among functional groups, fungal saprotrophs and pathotrophs represented by plant pathogens were significantly enriched in the early-successional stage, while fungal symbiotrophs represented by ectomycorrhiza were significantly increased in the late-successional stage. The abundance of both saprotroph and pathotroph fungal guilds was positively correlated with soil nitrate nitrogen and available phosphorus content. Ectomycorrhizal fungi were negatively correlated with nitrate nitrogen and available phosphorus content and positively correlated with ammonium nitrogen content. These results indicate that the dynamics of fungal community and function reflected the changes in nitrogen and phosphorus availability caused by the secondary succession in temperate forests. The fungal plant pathogen accumulated in the early-successional stage and ectomycorrhizal fungi accumulated in the late-successional stage may have a potential role in promoting forest succession. These findings contribute to a better understanding of the response of soil fungal communities to secondary forest succession and highlight the importance of fungal communities during the successional process.
土壤真菌群落在森林生态系统中发挥着重要作用，并受到森林次生演替的关键影响。然而，温带森林演替过程中真菌群落和功能的驱动因素及其对演替过程的潜在影响仍知之甚少。在本研究中，我们利用高通量DNA测序结合通过FUNGuild数据库进行功能预测，调查了三个温带森林次生演替阶段（灌丛、针叶林和落叶阔叶林）土壤真菌群落的动态变化。我们发现，在演替过程中真菌群落丰富度、α多样性和均匀度显著下降。初始演替发生后，土壤有效磷和硝态氮显著降低，冗余分析表明二者均是土壤真菌群落结构的重要预测因子。在功能组中，以植物病原体为代表的真菌腐生菌和致病菌在演替早期显著富集，而以外生菌根为代表的真菌共生菌在演替后期显著增加。腐生菌和致病菌真菌类群的丰度均与土壤硝态氮和有效磷含量呈正相关。外生菌根真菌与硝态氮和有效磷含量呈负相关，与铵态氮含量呈正相关。这些结果表明，真菌群落和功能的动态变化反映了温带森林次生演替引起的氮磷有效性变化。在演替早期积累的真菌植物病原体和在演替后期积累的外生菌根真菌可能在促进森林演替方面具有潜在作用。这些发现有助于更好地理解土壤真菌群落对次生森林演替的响应，并突出了真菌群落在演替过程中的重要性。
