Research Institute of Resource Insects, Chinese Academy of Forestry/Pu'er Forest Ecosystem Research Station, State Forestry Bureau, Kunming 650224, China.
Ying Yong Sheng Tai Xue Bao. 2021 Mar;32(3):887-894. doi: 10.13287/j.1001-9332.202103.039.
Soil bacterial diversity is a key factor for the maintanence of forest ecosystem function. Soil bacterial community would change along forest succession. We analyzed the variations of soil bacterial diversity and community composition at different successional stages in the forest, which would help understand the mechanism underlying forest restoration. We investigated soil bacterial diversity, community composition, and effect factors at different successional stages (including coniferous forest, mixed coniferous and broadleaf forest, and evergreen broadleaf forest) using Illumina Hiseq platform. The results showed that OTUs, Chao1 index, Ace index, and Shannon index of soil bacterial community decreased with the process of secondary succession. The highest soil bacterial total OTUs, richness, and complexity appeared at early successional stage. Soil bacterial community composition varied across different stages, with the mixed coniferous and broadleaf forest showing largest variation. Proteobacteria and Acidobacteria were common dominant phyla at secondary successional stages. Actinobacteria, Chloroflexi, and Patescibacteria were dominant phyla at the early successional stage, the abundance of which decreased with successional process in the forest. Proteobacteria and WPS-2 increased with the succession. Soil pH and tree species richness were key factors in driving soil bacterial community structure. The soil bacterial diversity decreased with forest succession, while the variations of soil bacterial community composition became larger.
土壤细菌多样性是维持森林生态系统功能的关键因素。土壤细菌群落会随着森林演替而发生变化。本研究分析了森林不同演替阶段土壤细菌多样性和群落组成的变化，这有助于理解森林恢复的机制。我们利用 Illumina Hiseq 平台研究了不同演替阶段（包括针叶林、针阔混交林和常绿阔叶林）的土壤细菌多样性、群落组成和影响因素。结果表明，土壤细菌群落的 OTUs、Chao1 指数、Ace 指数和 Shannon 指数随着次生演替过程而降低。土壤细菌总 OTUs、丰富度和复杂性在早期演替阶段最高。土壤细菌群落组成在不同阶段存在差异，其中针阔混交林变化最大。在次生演替阶段，变形菌门和酸杆菌门是常见的优势门。在早期演替阶段，放线菌门、绿弯菌门和 Patescibacteria 是优势门，其丰度随着森林演替过程而减少。变形菌门和 WPS-2 随着演替而增加。土壤 pH 和树种丰富度是驱动土壤细菌群落结构的关键因素。土壤细菌多样性随着森林演替而减少，而土壤细菌群落组成的变化变得更大。
