Shirai Ikuya, Iwasaki Yu, Karasawa Koji, Shigemura Yasutaka, Katayama Shigeru
Department of Science and Technology, Graduate School of Medicine, Science and Technology, Shinshu University, Nagano, Japan.
Ina Food Industry Co., Ltd., Nagano, Japan.
Front Nutr. 2023 Sep 25;10:1273328. doi: 10.3389/fnut.2023.1273328. eCollection 2023.
Agaro-oligosaccharides (AOSs) are known to have biological activities, such as anti-inflammatory, anti-tumor, and anti-obesity effects. Although existing evidence suggests the presence of AOSs in peripheral tissues after oral administration, whether AOSs permeate into the blood circulation remains unknown. Thus, we hypothesized that AOSs with low-molecular weight can permeate the human gastrointestinal tract. To test this hypothesis, the time course of absorption was examined by analyzing plasma samples before and 1, 2, and 4 h after ingestion. Analysis was performed using liquid chromatography/mass spectrometry after labeling with -aminobenzoic ethyl ester. Our results showed that the plasma concentration of agarobiose (Abi) was higher than that of agarotetraose (Ate); however, agarohexaose was not detected. Additionally, plasma levels of Abi and Ate were proportional to the dose. These results suggest that permeation efficiency is dependent on the molecular weight and that the systemic absorption of Abi via the gastrointestinal tract is better than that of Ate. These findings will contribute to a better understanding of the bioactivity of orally administered AOSs in peripheral tissues.
琼脂寡糖（AOSs）具有抗炎、抗肿瘤和抗肥胖等生物活性。尽管现有证据表明口服给药后外周组织中存在AOSs，但AOSs是否能渗透进入血液循环仍不清楚。因此，我们推测低分子量的AOSs能够渗透过人体胃肠道。为了验证这一假设，通过分析摄入前以及摄入后1、2和4小时的血浆样本，研究了吸收的时间进程。在用对氨基苯甲酸乙酯标记后，使用液相色谱/质谱进行分析。我们的结果表明，琼脂二糖（Abi）的血浆浓度高于琼脂四糖（Ate）；然而，未检测到琼脂六糖。此外，Abi和Ate的血浆水平与剂量成正比。这些结果表明渗透效率取决于分子量，并且Abi通过胃肠道的全身吸收优于Ate。这些发现将有助于更好地理解口服AOSs在外周组织中的生物活性。
