Wang Shiyu, Song Yi, He Zhiyong, Saneyoshi Hisao, Iwakiri Rie, Xu Pengyu, Zhao Chuanqi, Qu Xiaogang, Xu Yan
Division of Chemistry, Department of Medical Sciences, Faculty of Medicine, University of Miyazaki, 5200 Kihara, Kiyotake, Miyazaki 889-1692, Japan.
Shonan Laboratory, Corporate R&D Headquarters, Otsuka Chemical Co., Ltd., 2Chome26-1, Muraokahigashi, Fujisawa, Kanagawa 251-8555, Japan.
Chem Commun (Camb). 2023 Oct 24;59(85):12703-12706. doi: 10.1039/d3cc03192f.
The infectious disease coronavirus 2019 (SARS-CoV-2) is caused by a virus that has RNA as its genetic material. To understand the detailed structural features of SARS-COV-2 RNA, we probed the RNA structure by NMR. Two RNA sequences form a duplex and self-associate to form a dimeric G-quadruplex. The rG nucleoside was employed as a F sensor to confirm the RNA structure in cells by F NMR. A FRET assay further demonstrated that the dimeric G-quadruplex resulted in RNA dimerization in cells. These results provide the basis for the elucidation of SARS-COV-2 RNA function, which provides new insights into developing novel antiviral drugs against SARS-COV-2.
2019年冠状病毒病（SARS-CoV-2）这种传染病是由一种以RNA作为遗传物质的病毒引起的。为了解SARS-CoV-2 RNA的详细结构特征，我们通过核磁共振（NMR）对RNA结构进行了探测。两个RNA序列形成一个双链体并自缔合形成一个二聚体G-四链体。将核糖鸟苷（rG）核苷用作荧光传感器，通过荧光核磁共振（F NMR）在细胞中确认RNA结构。荧光共振能量转移（FRET）分析进一步证明，二聚体G-四链体导致细胞中的RNA二聚化。这些结果为阐明SARS-CoV-2 RNA功能提供了基础，这为开发针对SARS-CoV-2的新型抗病毒药物提供了新的见解。
