Department of General Gynecology, Women and Children's Hospital of Chongqing Medical University, Chongqing Health Center for Women and Children, Chongqing, 400037, People's Republic of China.
Hospital-Acquired Infection Control Department, Women and Children's Hospital of Chongqing Medical University, Chongqing Health Center for Women and Children, No. 120 Longshan Street, Yubei District, Chongqing, 400037, People's Republic of China.
Appl Biochem Biotechnol. 2024 Jul;196(7):4038-4048. doi: 10.1007/s12010-023-04742-0. Epub 2023 Oct 11.
Efficient isolation and sensitive quantification of Pseudomonas aeruginosa (P. aeruginosa) are crucial for identifying intrauterine infections and preventing the occurrence of intrauterine adhesion (IUA). However, traditional approaches, such as culture-based approach, are time-consuming. Herein, we constructed a detection scaffold by using primer exchange reaction (PER) that integrated the low-speed centrifugation-based isolation and sensitive quantification of target pathogenic bacteria. The established approach possesses several advantages, including (i) the approach is capable of simultaneous isolation and sensitive quantification of target bacteria; (ii) low-speed centrifugation or even manual equipment could be used to isolate target bacteria; and (iii) a low limit of detection was obtained as 54 cfu/mL. Based on this, the approach is a promising approach in analyzing P. aeruginosa from uterine secretions with IUA.
高效分离和灵敏定量铜绿假单胞菌（P. aeruginosa）对于鉴定宫内感染和预防宫腔粘连（IUA）的发生至关重要。然而，传统的方法，如基于培养的方法，耗时较长。在此，我们构建了一个基于引物交换反应（PER）的检测支架，该支架集成了基于低速离心的目标病原菌的分离和灵敏定量。所建立的方法具有以下几个优点：（i）该方法能够同时分离和灵敏定量目标细菌；（ii）可以使用低速离心甚至手动设备来分离目标细菌；（iii）检测限低至 54 cfu/mL。基于这一点，该方法有望用于分析患有 IUA 的子宫分泌物中的铜绿假单胞菌。
