Department of Chemistry, Physical and Theoretical Chemistry Laboratory, University of Oxford, South Parks Road, Oxford, OX1 3QZ, UK.
Chem Asian J. 2018 Oct 4;13(19):2758-2769. doi: 10.1002/asia.201800798. Epub 2018 Aug 24.
Bacterial infections represent one of the leading causes of mortality worldwide, nevertheless the design and development of rapid, cost-efficient and reliable detection methods for pathogens remains challenging. In recent years, electrochemical sensing methods have gained increasing attention for the detection of pathogenic bacteria, due to their increasingly competitive sensitivity. However, combining sensitivity with cost efficiency, high selectivity and a facile working procedure in a portable device is difficult. The presented review provides a summary of biosensing strategies for bacteria, published since 2015, by covering significant achievements towards custom-designed portable point-of-care devices. Herein, the direct chemical recognition of bacteria via enzyme activity or secretion products, as well as their detection at various electrode surfaces and materials, such as nanomaterials, indium tin oxide or paper-based immunosensors, is discussed. Furthermore, newly established hyphenated sensing principles, incorporated into lab-on-a-chip and microfluidic devices, are presented and remaining technical challenges and limitations are considered.
细菌感染是全球主要死亡原因之一，然而，设计和开发快速、经济高效且可靠的病原体检测方法仍然具有挑战性。近年来，由于电化学传感方法的灵敏度不断提高，因此越来越受到关注，可用于检测致病菌。然而，在便携式设备中结合灵敏度、成本效益、高选择性和简单的工作程序是困难的。本综述通过涵盖针对定制便携式即时检测设备的重大成就，总结了自 2015 年以来发表的用于细菌的生物传感策略。在此，通过酶活性或分泌产物的直接化学识别，以及在各种电极表面和材料（如纳米材料、氧化铟锡或基于纸张的免疫传感器）上的检测，讨论了对细菌的检测。此外，还介绍了新建立的组合传感原理，将其纳入芯片实验室和微流控设备中，并考虑了剩余的技术挑战和局限性。
