Kyungpook National University, Graduate School of Science and Technology, Department of Dental Hygiene, Sangju 37224, Republic of Korea.
Kyungpook National University, Research Center for Horse Industry, Department of Animal Science and Biotechnology, Sangju 37224, Republic of Korea.
J Appl Oral Sci. 2023 Oct 9;31:e20230243. doi: 10.1590/1678-7757-2023-0243. eCollection 2023.
Gossypetin, isolated from Hibiscus sabdariffa L, has been shown to have various pharmacological effects including anti-inflammatory and antibacterial activity against various diseases. However, since the effect of gossypetin in oral cancer remains to be reported, we aimed to investigate the anticancer activity and mechanisms of gossypetin in oral squamous cell carcinoma (OSCC).
The proliferation of OSCC cells was evaluated by cell viability and soft agar colony assays. The effects of gossypetin on the migration and invasion of OSCC cells was investigated by wound healing and transwell invasion assays, respectively. Apoptosis and cell cycle arrest were measured by flow cytometry. Moreover, the anticancer mechanism of gossypetin in OSCC cells was analyzed by western blotting.
Gossypetin inhibited the proliferation, migration, and invasion of OSCC cells and induced apoptosis by upregulating the Bax/Bcl-2 ratio and cell cycle arrest at the G2/M phase. Furthermore, gossypetin regulated the activation of extracellular signal-regulated kinase and nuclear factor-kappa B.
Results showed that gossypetin inhibits the proliferation, migration, and invasion of OSCC cells and triggers apoptosis and cell cycle arrest in OSCC. Therefore, gossypetin has the potential for use as a chemopreventive agent in oral cancer.
从木槿花中分离得到的棉子糖已显示出多种药理作用，包括对各种疾病的抗炎和抗菌活性。然而，由于棉子糖在口腔癌中的作用尚未报道，我们旨在研究棉子糖对口腔鳞状细胞癌（OSCC）的抗癌活性和机制。
通过细胞活力和软琼脂集落测定评估 OSCC 细胞的增殖。通过划痕愈合和 Transwell 侵袭测定分别研究棉子糖对 OSCC 细胞迁移和侵袭的影响。通过流式细胞术测量细胞凋亡和细胞周期停滞。此外，通过 Western blot 分析棉子糖在 OSCC 细胞中的抗癌机制。
棉子糖抑制 OSCC 细胞的增殖、迁移和侵袭，并通过上调 Bax/Bcl-2 比值和细胞周期阻滞在 G2/M 期诱导细胞凋亡。此外，棉子糖调节细胞外信号调节激酶和核因子-κB 的激活。
结果表明，棉子糖抑制 OSCC 细胞的增殖、迁移和侵袭，并触发 OSCC 中的细胞凋亡和细胞周期停滞。因此，棉子糖具有作为口腔癌化学预防剂的潜力。
