Omori Haruka, Shan Qiusheng, Takabatake Kiyofumi, Nakano Keisuke, Kawai Hotaka, Sukegawa Shintaro, Tsujigiwa Hidetsugu, Nagatsuka Hitoshi
Department of Oral Pathology and Medicine, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Science, Okayama 700-8525, Japan.
Department of Oral and Maxillofacial Surgery, Kagawa Prefectural Central Hospital, Kagawa 760-0065, Japan.
Cancers (Basel). 2021 Jul 12;13(14):3491. doi: 10.3390/cancers13143491.
Normal stromal cells surrounding the tumor parenchyma, such as the extracellular matrix (ECM), normal fibroblasts, mesenchymal stromal cells, and osteoblasts, play a significant role in the progression of cancers. However, the role of gingival and periodontal ligament tissue-derived stromal cells in OSCC progression is unclear. In this study, the effect of G-SCs and P-SCs on the differentiation, proliferation, invasion, and migration of OSCC cells in vitro was examined by Giemsa staining, Immunofluorescence (IF), (3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium) (MTS), invasion, and migration assays. Furthermore, the effect of G-SCs and P-SCs on the differentiation, proliferation, and bone invasion by OSCC cells in vivo was examined by hematoxylin-eosin (HE) staining, immunohistochemistry (IHC), and tartrate-resistant acid phosphatase (TRAP) staining, respectively. Finally, microarray data and bioinformatics analyses identified potential genes that caused the different effects of G-SCs and P-SCs on OSCC progression. The results showed that both G-SCs and P-SCs inhibited the differentiation and promoted the proliferation, invasion, and migration of OSCC in vitro and in vivo. In addition, genes, including , , , , , and , are probably involved in causing the different effects of G-SCs and P-SCs on OSCC progression. Therefore, as a potential regulatory mechanism, both G-SCs and P-SCs can promote OSCC progression.
肿瘤实质周围的正常基质细胞，如细胞外基质（ECM）、正常成纤维细胞、间充质基质细胞和成骨细胞，在癌症进展中发挥着重要作用。然而，牙龈和牙周韧带组织来源的基质细胞在口腔鳞状细胞癌（OSCC）进展中的作用尚不清楚。在本研究中，通过吉姆萨染色、免疫荧光（IF）、（3-（4,5-二甲基噻唑-2-基）-5-（3-羧甲氧基苯基）-2-（4-磺基苯基）-2H-四唑）（MTS）、侵袭和迁移试验，检测了牙龈来源的基质细胞（G-SCs）和牙周韧带来源的基质细胞（P-SCs）对OSCC细胞体外分化、增殖、侵袭和迁移的影响。此外，分别通过苏木精-伊红（HE）染色、免疫组织化学（IHC）和抗酒石酸酸性磷酸酶（TRAP）染色，检测了G-SCs和P-SCs对OSCC细胞体内分化、增殖和骨侵袭的影响。最后，通过微阵列数据和生物信息学分析，确定了导致G-SCs和P-SCs对OSCC进展产生不同影响的潜在基因。结果表明，G-SCs和P-SCs在体外和体内均抑制OSCC的分化，并促进其增殖、侵袭和迁移。此外，包括[此处原文缺失具体基因名称]等基因可能参与导致G-SCs和P-SCs对OSCC进展产生不同影响。因此，作为一种潜在的调节机制，G-SCs和P-SCs均可促进OSCC进展。
