Turk Ahmet, Kuloglu Tuncay, Karadag Abdullah, Ozcan Metin Tuba
Department of Histology and Embryology, Faculty of Medicine, Adiyaman University, Adiyaman, TUR.
Department of Histology and Embryology, Faculty of Medicine, Firat University, Elazig, TUR.
Cureus. 2023 Oct 9;15(10):e46711. doi: 10.7759/cureus.46711. eCollection 2023 Oct.
Background Cyclophosphamide (CP), commonly used as an anticarcinogenic drug, has the potential to induce detrimental effects on multiple tissues, including the liver. Asprosin, which is a glucogenic adipokine, induces the liver to secrete glucose, thus contributing to the maintenance of homeostasis. This study aims to investigate the immunoreactivity of asprosin in the liver tissue of rats exposed to CP administration, as well as the changes in its levels due to the supplementation of Vitamin D (Vit D). Materials and methods Four experimental groups were formed, including control, Vit D (200 IU/kg), CP (200 mg/kg), and Vit D+ CP. Histopathological analysis was carried out by employing staining methods on liver tissues. These techniques encompassed the application of hematoxylin-eosin (H&E), Masson's trichrome, and periodic acid Schiff (PAS). Through the application of spectrophotometric methods, concentrations of malondialdehyde (MDA), total antioxidant status (TAS), total oxidant status (TOS), and asprosin were determined. Furthermore, apoptotic cells were identified by the terminal deoxynucleotidyl transferase (TdT) dUTP nick-end labeling (TUNEL) method, and the asprosin immunoreactivity was determined by immunohistochemistry. Results Under light microscope examination, the histopathological damage was found to be more notable in the CP group compared to the control group. Moreover, a decrease was observed in serum and tissue asprosin levels, while an increase was noted in the count of apoptotic cells, along with elevated MDA and TOS levels. However, in the CP+Vit D group, Vit D administration alleviated histopathological damage. Notably, there were significant increases in TAS and asprosin levels, accompanied by reductions in both MDA and TOS levels. Conclusions The effect of CP on liver tissue was observed to result in damage and a reduction in asprosin levels. Vit D supplementation revealed elevated asprosin levels and a distinct protective effect on the tissue. Considering the association between asprosin and liver injury induced by CP, further research is needed to elucidate the mechanisms that underlie the effect of asprosin on tissues. When combined with Vit D, asprosin holds promise for potential clinical applications as a therapeutic target.
背景 环磷酰胺（CP）常用作抗癌药物，有对包括肝脏在内的多种组织产生有害影响的可能性。Asprosin是一种生糖脂肪因子，可诱导肝脏分泌葡萄糖，从而有助于维持体内平衡。本研究旨在调查接受CP给药的大鼠肝脏组织中Asprosin的免疫反应性，以及补充维生素D（Vit D）后其水平的变化。材料与方法 组建了四个实验组，包括对照组、Vit D（200 IU/kg）组、CP（200 mg/kg）组和Vit D + CP组。通过对肝脏组织采用染色方法进行组织病理学分析。这些技术包括苏木精-伊红（H&E）染色、Masson三色染色和过碘酸希夫（PAS）染色。通过分光光度法测定丙二醛（MDA）、总抗氧化状态（TAS）、总氧化状态（TOS）和Asprosin的浓度。此外，通过末端脱氧核苷酸转移酶（TdT）介导的dUTP缺口末端标记（TUNEL）法鉴定凋亡细胞，并通过免疫组织化学测定Asprosin的免疫反应性。结果 在光学显微镜检查下，发现CP组的组织病理学损伤比对照组更明显。此外，血清和组织中Asprosin水平降低，而凋亡细胞计数增加，同时MDA和TOS水平升高。然而，在CP + Vit D组中，给予Vit D减轻了组织病理学损伤。值得注意的是，TAS和Asprosin水平显著升高，同时MDA和TOS水平降低。结论 观察到CP对肝脏组织的作用导致损伤并降低了Asprosin水平。补充Vit D显示出Asprosin水平升高以及对组织有明显保护作用。考虑到Asprosin与CP诱导的肝损伤之间的关联，需要进一步研究以阐明Asprosin对组织产生作用的潜在机制。当与Vit D联合使用时，Asprosin有望作为治疗靶点用于潜在的临床应用。
