Marefati Narges, Beheshti Farimah, Anaeigoudari Akbar, Alipour Fatemeh, Shafieian Reyhaneh, Akbari Fatemeh, Pirasteh Maryam, Mahmoudabady Maryam, Salmani Hossein, Mawdodi Sara, Hosseini Mahmoud
Department of Physiology and Medical Physics, Faculty of Medicine, Baqiyatallah University of Medical Sciences, Tehran, Iran.
Applied Biomedical Research Center, Mashhad University of Medical Sciences, Mashhad, Iran.
J Cardiovasc Thorac Res. 2023;15(2):106-115. doi: 10.34172/jcvtr.2023.31719. Epub 2023 Jun 29.
Inflammation and oxidative stress are contributed to cardiovascular diseases. Vitamin D (Vit D) has antioxidant and anti-inflammatory properties. In the current research, the effect of Vit D on cardiac fibrosis and inflammation, and oxidative stress indicators in cardiovascular tissues was studied in lipopolysaccharides(LPS) injected rats.
Rats were distributed into 5 groups and were treated for 2 weeks. Control: received vehicle(saline supplemented with tween-80) instead of Vit D and saline instead of LPS, LPS: treated by 1 mg/kg of LPS and was given vehicle instead of Vit D, LPS-Vit D groups: received 3 doses of Vit D (100, 1000, and 10000 IU/kg) of Vit D in addition to LPS. Vit D was dissolved in saline supplemented with tween-80 (final concentration 0.1%) and LPS was dissolved in saline. The white blood cell (WBC) was counted. Oxidative stress markers were determined in serum, aorta, and heart. Cardiac tissue fibrosis was also estimated using Masson's trichrome staining method.
WBC and malondialdehyde (MDA) were higher in the LPS group than the control group, whereas the thiol content, superoxide dismutase (SOD), and catalase (CAT) were lower in the LPS group than the control group (<0.01 and <0.001). Administration of Vit D decreased WBC (<0.001) and MDA (<0.05 and <0.001) while enhanced thiol (dose 10000 IU/Kg) (<0.001), SOD (dose 10000 IU/kg) (<0.001), and CAT (<0.05 and <0.001) compared to the LPS group. All doses of Vit D also decreased cardiac fibrosis compared to the LPS group (<0.001).
Vit D protected the cardiovascular against the detrimental effect of LPS. This cardiovascular protection can be attributed to the antioxidant and anti-inflammatory properties of Vit D.
炎症和氧化应激与心血管疾病的发生有关。维生素D（Vit D）具有抗氧化和抗炎特性。在本研究中，我们在注射脂多糖（LPS）的大鼠中研究了Vit D对心脏纤维化、炎症以及心血管组织中氧化应激指标的影响。
将大鼠分为5组并进行2周的治疗。对照组：接受溶剂（添加吐温-80的生理盐水）而非Vit D，以及生理盐水而非LPS；LPS组：用1mg/kg的LPS进行处理，并给予溶剂而非Vit D；LPS-Vit D组：除LPS外，接受3种剂量的Vit D（100、1000和10000IU/kg）。Vit D溶解于添加吐温-80的生理盐水中（终浓度0.1%），LPS溶解于生理盐水中。计数白细胞（WBC）。测定血清、主动脉和心脏中的氧化应激标志物。还使用Masson三色染色法评估心脏组织纤维化。
LPS组的WBC和丙二醛（MDA）高于对照组，而LPS组的硫醇含量、超氧化物歧化酶（SOD）和过氧化氢酶（CAT）低于对照组（<0.01和<0.001）。与LPS组相比，给予Vit D可降低WBC（<0.001）和MDA（<0.05和<0.001），同时提高硫醇（10000IU/Kg剂量）（<0.001）、SOD（10000IU/kg剂量）（<0.001）和CAT（<0.05和<0.001）。与LPS组相比，所有剂量的Vit D还可降低心脏纤维化（<0.001）。
Vit D可保护心血管免受LPS的有害影响。这种心血管保护作用可归因于Vit D的抗氧化和抗炎特性。
