Universidade Federal de Minas Gerais, Faculdade de Odontologia Departamento de Clínica, Patologia e Cirurgia Odontológicas Av. Antônio Carlos, 6627, Pampulha, Belo Horizonte, MG CEP: 31.270-901, Brasil
Med Oral Patol Oral Cir Bucal. 2023 Nov 1;28(6):e512-e518. doi: 10.4317/medoral.25861.
Oral Lichen Planus is a potential malignant disorder and shares clinical and histopathological features with other similar lesions. ALDH1 is a specific biomarker for stem cells identification, however its role in stromal cells of immune inflammatory infiltrate has not been explored. The aim of this study was to investigate the ALDH1 immunoexpression in epithelial and stromal cells of Oral Lichen Planus and other lesions with lichenoid inflammatory infiltrate.
64 samples of Oral Lichen Planus, Oral Lichenoid Lesions, Oral Leukoplakia and Unspecific Chronic Inflammation were included. ALDH1 was evaluated in both epithelium and stromal cells. ALDH1+ cells ≥ 5% were considered positive in epithelium. Stromal cells were evaluated semi quantitatively. Fields were ranked in scores, according to criteria: 1 (0 to 10%); 2 (11 to 50%) and 3 (>50%). The mean value of the sum of the fields was the final score. Statistical differences among groups were investigated, considering p < 0.05.
ALDH1 expression in epithelium was low in all groups without difference among them. ALDH1+ cells in the lamina propria were higher for Lichen Planus [2.0], followed by Leukoplakia [1.3], Lichenoid lesions [1.2] and control [1.1] (p<0.05).
ALDH1 immunoexpression in epithelium of lichenoid potential malignant disorders did not show a contributory tool, however ALDH1 in stromal cells of lichen planus might be involved in the complex process of immune regulation associated with the pathogenesis of this disease.
口腔扁平苔藓是一种潜在的恶性疾病，与其他类似病变具有临床和组织病理学特征。ALDH1 是干细胞鉴定的特异性生物标志物，但它在免疫炎症浸润的间质细胞中的作用尚未被探索。本研究旨在探讨 ALDH1 在口腔扁平苔藓和其他具有苔藓样炎症浸润的病变的上皮和间质细胞中的免疫表达。
纳入 64 例口腔扁平苔藓、口腔苔藓样病变、口腔白斑和非特异性慢性炎症的样本。评估上皮和间质细胞中的 ALDH1。上皮细胞中 ALDH1+细胞≥5%为阳性。间质细胞进行半定量评估。根据标准将视野评分分为 1 分（0 至 10%）、2 分（11 至 50%）和 3 分（>50%）。各视野得分的平均值为最终得分。考虑到 p<0.05，研究了各组之间的统计学差异。
所有组的上皮细胞中 ALDH1 表达均较低，各组之间无差异。固有层中的 ALDH1+细胞在扁平苔藓中较高[2.0]，其次是白斑[1.3]、苔藓样病变[1.2]和对照组[1.1]（p<0.05）。
苔藓样潜在恶性疾病的上皮细胞中 ALDH1 的免疫表达并未显示出辅助作用，然而，扁平苔藓的间质细胞中的 ALDH1 可能参与与该疾病发病机制相关的复杂免疫调节过程。
