Department of Oral Pathology and Microbiology, Educare Institute of Dental Sciences, College Road, Kiliyamannil Campus, Chattiparamba, Malappuram, Kerala, India.
Department of Oral Pathology and Microbiology, Coorg Institute of Dental Sciences, Kodagu, Karnataka, India.
Indian J Pathol Microbiol. 2023 Jan-Mar;66(1):9-13. doi: 10.4103/ijpm.ijpm_864_21.
Oral lichen planus is a T-cell-mediated chronic inflammatory disease affecting approximately 1% to 2% of the population, the etiology of which is currently unknown. The objectives of this study were to observe if senescence occurs in oral lichen planus, through the assessment of the immunohistochemical expression of a novel marker for senescence called Senescence marker protein-30 or regucalcin, and compare the expression to that in oral lichenoid reaction and non-specific inflammation.
The study material consisted of 30 cases of oral lichen planus, 15 cases of oral lichenoid reaction and 15 cases of non-specific inflammation. The number of positive cells in ten randomly selected high power fields were counted in the epithelium and the connective tissue separately and the mean was determined.
Mann-Whitney U test was used to statistically analyze if there was any significant difference in the expression of Senescence marker protein-30 between oral lichen planus, oral lichenoid reaction and non-specific inflammation. Even though a greater expression was seen in the oral lichen planus cases than oral lichenoid reaction, the difference in both the epithelium and connective tissue was not statistically significant.
This study shows that in addition to the already known mechanisms like apoptosis and increased cell proliferation rates, the activated T-lymphocytes may also trigger a senescent change in the cells of oral lichen planus. As with the other mechanisms, this is also seen only in a small proportion of the cases.
口腔扁平苔藓是一种 T 细胞介导的慢性炎症性疾病，影响约 1%至 2%的人口，其病因目前尚不清楚。本研究的目的是通过评估一种称为衰老标志物蛋白-30 或钙调节蛋白的新型衰老标志物的免疫组织化学表达，观察口腔扁平苔藓是否发生衰老，并将其与口腔扁平苔藓样反应和非特异性炎症进行比较。
研究材料包括 30 例口腔扁平苔藓、15 例口腔扁平苔藓样反应和 15 例非特异性炎症。在十个随机选择的高倍视野中分别对上皮和结缔组织中的阳性细胞数进行计数，并确定平均值。
采用曼-惠特尼 U 检验统计分析口腔扁平苔藓、口腔扁平苔藓样反应和非特异性炎症之间 Senescence marker protein-30 表达是否存在显著差异。尽管口腔扁平苔藓病例的表达高于口腔扁平苔藓样反应，但上皮和结缔组织中的差异均无统计学意义。
本研究表明，除了已知的凋亡和细胞增殖率增加等机制外，活化的 T 淋巴细胞也可能引发口腔扁平苔藓细胞的衰老变化。与其他机制一样，这种情况也仅见于一小部分病例。
