Guangdong and Shenzhen Key Laboratory of Reproductive Medicine and Genetics, Institute of Urology, Peking University Shenzhen Hospital, Shenzhen Peking University-The Hong Kong University of Science and Technology Medical Center, Shenzhen, China.
State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, China.
FASEB J. 2023 Nov;37(11):e23244. doi: 10.1096/fj.202301278.
The mitotic quiescence of prospermatogonia is the event known to occur during genesis of the male germline and is tied to the development of the spermatogenic lineage. The regulatory mechanisms and the functional importance of this process have been demonstrated in mice; however, regulation of this process in human and domestic animal is still largely unknown. In this study, we employed single-cell RNA sequencing to identify transcriptional signatures of prospermatogonia and major somatic cell types in testes of goats at E85, E105, and E125. We identified both common and specific Gene Ontology categories, transcription factor regulatory networks, and cell-cell interactions in cell types from goat testis. We also analyzed the transcriptional dynamic changes in prospermatogonia, Sertoli cells, Leydig cells, and interstitial cells. Our datasets provide a useful resource for the study of domestic animal germline development.
精原细胞有丝分裂静止是已知发生在雄性生殖系发生过程中的事件，与精子发生谱系的发育有关。这一过程的调节机制和功能重要性已在小鼠中得到证实；然而，人类和家畜中这一过程的调节仍在很大程度上未知。在这项研究中，我们采用单细胞 RNA 测序来鉴定 E85、E105 和 E125 时期山羊睾丸中精原细胞和主要体细胞类型的转录特征。我们鉴定了山羊睾丸细胞类型中的常见和特定的基因本体类别、转录因子调控网络和细胞-细胞相互作用。我们还分析了精原细胞、支持细胞、间质细胞和间质细胞中基因表达的动态变化。我们的数据集为研究家畜生殖系发育提供了有用的资源。
