Pediatrics, Department, Faculty of Medicine, Zagazig University, Zagazig, 44519, Egypt.
Ultra Medical Center, Alain-Abu Dhabi, United Arab Emirates.
Eur J Pediatr. 2023 Dec;182(12):5673-5679. doi: 10.1007/s00431-023-05242-3. Epub 2023 Oct 12.
Though pathogenesis of primary immune thrombocytopenia (ITP) is still rendered unclear, yet there are many research efforts that have been directed to the role of T helper 17 (Th17) and interleukin 17 (IL-17) in the pathogenesis of this disease. The Th17 cell, which produces IL-17, is a subset of T helper cells. Interleukin 17 is pro-inflammatory cytokine that is recently proved to have a crucial role in the emergence of autoimmune diseases. We aimed to investigate the role of T helper17 cells and interleukin-17 in the pathogenesis of ITP in Egyptian children. This study was carried out on 100 children with ITP and 100 apparently healthy children as a control group. Patients were subjected to full medical history taking, thorough physical examination and routine investigations according to our local standards. Percentage of Th17 cells was measured by flow cytometry in study groups. Also, serum IL-17 was measured in in study groups by ELISA. Th 17 cells were significantly higher in patients compared to controls. Moreover, 3.1-fold increased serum levels of IL-17 were observed in patients with ITP compared to controls. Newly diagnosed patients had significantly higher percentage of Th-17cells as well as higher IL-17 levels than patients with either persistent or chronic ITP. Conclusion: We concluded that Th 17 cells and IL-17 seem to play an important role in the pathogenesis of ITP in Egyptian children. What is Known -- What is New: • The pathogenesis of ITP is heterogeneous A novel subset of CD4+ T cells, distinct from Th1 and Th2, was recently identified. It is characterized by the production of IL-17 and, therefore, designated as Th17 cells. Several studies support a pivotal role for serum cytokines in the pathogenesis of ITP and provide evidence to suggest that helper Tlymphocytes polarize into Th1 and Th2 immune response. we aimed to investigate the role of T helper17 cells and interleukin-17 in the pathogenesis of ITP in Egyptian children.
虽然原发性免疫性血小板减少症 (ITP) 的发病机制仍不清楚，但已有许多研究致力于 Th17 细胞和白细胞介素 17 (IL-17) 在该病发病机制中的作用。Th17 细胞是产生白细胞介素 17 的 T 辅助细胞亚群。白细胞介素 17 是一种促炎细胞因子，最近被证明在自身免疫性疾病的发生中具有关键作用。我们旨在研究 Th17 细胞和白细胞介素-17 在埃及儿童 ITP 发病机制中的作用。本研究共纳入 100 例 ITP 患儿和 100 例健康儿童作为对照组。根据当地标准，对患者进行详细的病史询问、全面的体格检查和常规检查。通过流式细胞术测量研究组 Th17 细胞的百分比。同时，通过 ELISA 测量研究组血清中的白细胞介素-17。与对照组相比，患者 Th17 细胞明显升高。此外，与对照组相比，ITP 患者的血清白细胞介素-17 水平升高了 3.1 倍。新诊断的患者的 Th-17 细胞百分比以及白细胞介素-17 水平均明显高于持续性或慢性 ITP 患者。结论：我们得出结论，Th17 细胞和白细胞介素-17 似乎在埃及儿童 ITP 的发病机制中起重要作用。已知的-新的：• ITP 的发病机制是异质的最近发现了一种新型的 CD4+T 细胞亚群，与 Th1 和 Th2 不同，其特征是产生白细胞介素-17，因此被命名为 Th17 细胞。几项研究支持细胞因子在 ITP 发病机制中的重要作用，并提供证据表明辅助性 T 淋巴细胞向 Th1 和 Th2 免疫反应分化。我们旨在研究 Th17 细胞和白细胞介素-17 在埃及儿童 ITP 发病机制中的作用。
