Department of Chemistry, Purdue University, West Lafayette, IN, 47907, United States.
Department of Chemistry, Purdue University, West Lafayette, IN, 47907, United States.
Anal Chim Acta. 2023 Oct 23;1279:341830. doi: 10.1016/j.aca.2023.341830. Epub 2023 Sep 17.
Nanospray desorption electrospray ionization (nano-DESI) is an ambient ionization technique that enables molecular imaging of biological samples with high spatial resolution. We have recently developed an integrated microfluidic probe (iMFP) for nano-DESI mass spectrometry imaging (MSI) that significantly enhances the robustness of the technique. In this study, we designed a new probe that enables imaging of biological samples with high spatial resolution. The new probe design features smaller primary and spray channels and an entirely new configuration of the sampling port that enables robust imaging of tissues with a spatial resolution of 8-10 μm. We demonstrate the spatial resolution, sensitivity, durability, and throughput of the iMFP by imaging mouse uterine and brain tissue sections. The robustness of the high-resolution iMFP allowed us to perform first imaging experiments with both high spatial resolution and high throughput, which is particularly advantageous for high-resolution imaging of large tissue sections of interest to most MSI applications. Overall, the new probe design opens opportunities for mapping of biomolecules in biological samples with high throughput and cellular resolution, which is important for understanding biological systems.
纳米喷雾解吸电喷雾电离（nano-DESI）是一种环境电离技术，可实现具有高空间分辨率的生物样品的分子成像。我们最近开发了一种用于 nano-DESI 质谱成像（MSI）的集成微流控探头（iMFP），该探头显著提高了该技术的稳健性。在这项研究中，我们设计了一种新的探头，可实现具有高空间分辨率的生物样品成像。新探头设计的特点是更小的主通道和喷雾通道，以及采样端口的全新配置，可实现具有 8-10μm 空间分辨率的组织的稳健成像。我们通过对小鼠子宫和脑组织切片进行成像，展示了 iMFP 的空间分辨率、灵敏度、耐用性和通量。高分辨率 iMFP 的稳健性使我们能够进行具有高空间分辨率和高通量的首次成像实验，这对于大多数 MSI 应用中对高分辨率成像的大型组织切片尤其有利。总的来说，新探头设计为具有高通量和细胞分辨率的生物样品中的生物分子成像开辟了机会，这对于理解生物系统很重要。
