Shang Zhuye, Meng Qingtao, Zhang Run, Zhang Zhiqiang
School of Chemical Engineering, University of Science and Technology Liaoning, Anshan, Liaoning Province, 114051, PR China.
School of Chemical Engineering, University of Science and Technology Liaoning, Anshan, Liaoning Province, 114051, PR China; Key Laboratory for Functional Material, Educational Department of Liaoning Province, University of Science and Technology Liaoning, Anshan, Liaoning Province, 114051, PR China.
Anal Chim Acta. 2023 Oct 23;1279:341783. doi: 10.1016/j.aca.2023.341783. Epub 2023 Sep 5.
We report the development of a bifunctional near-infrared fluorescent probe (QZB) for selective sensing of bisulfite (HSO) and hypochlorous acid (HOCl). The synergistic detection of HSO and HOCl was achieved via a C=C bond recognition site. In comparison with the red-fluorescence QZB, two different products with non-fluorescence and paleturquoise fluorescence were produced by the recognition of QZB towards HSO and HOCl respectively, which can realize effectively the dual-functional detection of HSO and HOCl. QZB features prominent preponderances of dual-function response, near-infrared emission, reliability at physiological pH, low cytotoxicity and high sensitivity to HSO and HOCl. The detection of HSO in actual food samples has been successfully achieved using QZB. Utilization of QZB-based test strip to semi-quantitatively detect HSO and HOCl in real-world water samples by the "naked-eye" colorimetry are then demonstrated. Simultaneously, the determination of HSO and HOCl in real-world water sample has been achieved by smartphone-based standard curves. Additionally, the applications of QZB for imaging HSO and HOCl in vivo are successfully demonstrated. Consequently, the successful development of QZB could be promising as an efficient tool for researching the role of HSO/HOCl in the regulation of redox homeostasis regulation in vivo and complex signal transduction and for future food safety evaluation.
我们报道了一种用于选择性检测亚硫酸氢根（HSO₃⁻）和次氯酸（HOCl）的双功能近红外荧光探针（QZB）的研发。通过碳碳双键识别位点实现了对HSO₃⁻和HOCl的协同检测。与红色荧光的QZB相比，QZB分别与HSO₃⁻和HOCl识别产生两种不同产物，即无荧光产物和淡蓝绿色荧光产物，可有效实现对HSO₃⁻和HOCl的双功能检测。QZB具有双功能响应、近红外发射、在生理pH值下稳定、低细胞毒性以及对HSO₃⁻和HOCl高灵敏度等突出优势。使用QZB已成功实现对实际食品样品中HSO₃⁻的检测。随后展示了利用基于QZB的试纸条通过“肉眼”比色法对实际水样中的HSO₃⁻和HOCl进行半定量检测。同时，通过基于智能手机的标准曲线实现了对实际水样中HSO₃⁻和HOCl的测定。此外，还成功证明了QZB在体内对HSO₃⁻和HOCl成像的应用。因此，QZB的成功研发有望成为研究HSO₃⁻/HOCl在体内氧化还原稳态调节和复杂信号转导中的作用以及未来食品安全评估的有效工具。
