Jiang Jian, Wang Shaocai, Wang Sai, Yang Yinshuang, Zhang Xiuli, Wang Wenjun, Zhu Xu, Fang Mingxi, Xu Yaozeng
Department of Orthopaedics, The First Affiliated Hospital of Soochow University, Suzhou 215006, China.
Department of Orthopaedics, The Affiliated Hospital of Xuzhou Medical University, Xuzhou, China.
Anal Methods. 2023 Jul 6;15(26):3188-3195. doi: 10.1039/d3ay00338h.
The phagocyte's lysosome is the primary site of hypochlorous acid (HOCl) synthesis, and HOCl can be used as a biomarker for osteoarthritis diagnosis and treatment evaluation. Accurate detection of HOCl with high sensitivity and selectivity is required to understand its activities in healthy bio-systems and diseases. By integrating acceptable design principles and dye screening methodologies, we proposed and developed a novel near-infrared fluorescent HOCl sensing probe (FNIR-HOCl). The FNIR-HOCl probe has a quick reaction rate, high sensitivity (LOD = 70 nM), and excellent selectivity toward HOCl over other metal ions and reactive oxygen species. It has been successfully implemented to detect endogenous HOCl produced by RAW264.7 cells, as well as imaging towards mice with osteoarthritis. As a result, the probe FNIR-HOCl is extremely promising as a biological tool for revealing the roles of HOCl in various physiological and pathological contexts.
吞噬细胞的溶酶体是次氯酸（HOCl）合成的主要场所，并且HOCl可用作骨关节炎诊断和治疗评估的生物标志物。为了解其在健康生物系统和疾病中的活性，需要高灵敏度和高选择性地准确检测HOCl。通过整合可接受的设计原则和染料筛选方法，我们提出并开发了一种新型近红外荧光HOCl传感探针（FNIR-HOCl）。FNIR-HOCl探针反应速率快、灵敏度高（检测限=70 nM），对HOCl的选择性优于其他金属离子和活性氧。它已成功用于检测RAW264.7细胞产生的内源性HOCl，以及对骨关节炎小鼠进行成像。因此，作为一种揭示HOCl在各种生理和病理环境中作用的生物工具，探针FNIR-HOCl极具前景。
