通过计算机辅助酶联免疫吸附测定法测量细胞培养上清液中的免疫球蛋白浓度。

Measurement of immunoglobulin concentration in cell culture supernates by computer-assisted ELISA.

作者信息

Slade H B, Lopatin D E, Schwartz S A

出版信息

J Immunol Methods. 1986 Nov 20;94(1-2):169-79. doi: 10.1016/0022-1759(86)90230-9.

Abstract

The enzyme-linked immunosorbent assay (ELISA) is used extensively in immunologic research for obtaining quantitative estimates of immunoglobulin concentration in cell culture supernates. Through incorporation of a microcomputer for data acquisition, storage and rapid calculation of results, a substantial reduction in total assay time may be realized. Described here are a set of menu-driven programs written in Basic for the IBM-PC which provide advantages over existing software in simplicity, versatility and accuracy. Hardware requirements are minimal. These programs should encourage greater flexibility in terms of the size and complexity of experimental designs.

摘要

酶联免疫吸附测定法（ELISA）在免疫研究中被广泛用于定量估计细胞培养上清液中的免疫球蛋白浓度。通过引入微型计算机进行数据采集、存储和结果的快速计算，可以大幅缩短总测定时间。本文介绍了一组用Basic语言为IBM-PC编写的菜单驱动程序，这些程序在简单性、通用性和准确性方面优于现有软件。硬件要求极低。这些程序应能在实验设计的规模和复杂性方面提供更大的灵活性。