Lai Siyi, Wang Shengnian, Luo Jun, Lee L James, Yang Shang-Tian, Madou Marc J
Department of Chemical Engineering, Ohio State University, 140 West 19th Avenue, Columbus, OH 43210, USA.
Anal Chem. 2004 Apr 1;76(7):1832-7. doi: 10.1021/ac0348322.
This paper presents an integrated microfluidic device on a compact disk (CD) that performs an enzyme-linked immunosorbent assay (ELISA) for rat IgG from a hybridoma cell culture. Centrifugal and capillary forces were used to control the flow sequence of different solutions involved in the ELISA process. The microfluidic device was fabricated on a plastic CD. Each step of the ELISA process was carried out automatically by controlling the rotation speed of the CD. The work on analysis of rat IgG from hybridoma culture showed that the microchip-based ELISA has the same detection range as the conventional method on the 96-well microtiter plate but has advantages such as less reagent consumption and shorter assay time over the conventional method.
本文介绍了一种集成在光盘（CD）上的微流控装置，该装置可对杂交瘤细胞培养物中的大鼠IgG进行酶联免疫吸附测定（ELISA）。利用离心力和毛细管力来控制ELISA过程中不同溶液的流动顺序。该微流控装置制作在塑料CD上。通过控制CD的转速，ELISA过程的每一步都能自动进行。对杂交瘤培养物中大鼠IgG的分析工作表明，基于微芯片的ELISA与传统的96孔微量滴定板方法具有相同的检测范围，但与传统方法相比，具有试剂消耗少和检测时间短等优点。
