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一种经过改良的酶联免疫吸附测定法,可选择性检测识别天然抗原的单克隆抗体。

A modified ELISA that selectively detects monoclonal antibodies recognizing native antigen.

作者信息

Smith A D, Wilson J E

出版信息

J Immunol Methods. 1986 Nov 20;94(1-2):31-5. doi: 10.1016/0022-1759(86)90212-7.

DOI:10.1016/0022-1759(86)90212-7
PMID:3782816
Abstract

A modified ELISA procedure is described which permits selective detection of monoclonal antibodies reactive with the native antigen. The essence of the method is the use of immobilization methods that do not result in denaturation of the antigen during adsorption to the microtiter plates. This was accomplished by using polyclonal antibodies, adsorbed to the wells of the microtiter plate, as the immobilizing agent.

摘要

本文描述了一种改良的酶联免疫吸附测定(ELISA)方法,该方法可选择性检测与天然抗原反应的单克隆抗体。该方法的核心是采用固定方法,使抗原在吸附到微量滴定板过程中不会变性。这是通过使用吸附在微量滴定板孔中的多克隆抗体作为固定剂来实现的。

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