一种用于检测和鉴定针对鼠抗人 HLA 和肿瘤相关抗原单克隆抗体的同基因抗独特型抗体的夹心测定法。
A sandwich assay to detect and characterize syngeneic anti-idiotypic antibodies to murine anti-HLA and tumor associated antigen monoclonal antibodies.
作者信息
Tsujisaki M, Kusama M, Sakaguchi K, Perosa F, Ferrone S
出版信息
J Immunol Methods. 1986 Dec 4;95(1):47-55. doi: 10.1016/0022-1759(86)90316-9.
The parameters of a sandwich assay to analyze syngeneic polyclonal and monoclonal anti-idiotypic antibodies to murine anti-HLA and anti-human melanoma associated antigen monoclonal antibodies have been characterized. Furthermore, the sensitivity of the sandwich assay has been compared with that of assays widely used to characterize anti-idiotypic antibodies, i.e., the binding assay to F(ab')2 fragments of monoclonal antibodies and the radioimmunoassay. The latter is more sensitive than the sandwich assay when monoclonal anti-idiotypic antibodies are tested, but less sensitive when anti-idiotypic antisera are analyzed. Both assays are less sensitive than the binding assay to F(ab')2 fragments of monoclonal antibodies.
已对用于分析针对鼠抗人 HLA 和抗人黑素瘤相关抗原单克隆抗体的同基因多克隆和单克隆抗独特型抗体的夹心测定参数进行了表征。此外，还将夹心测定的灵敏度与广泛用于表征抗独特型抗体的测定方法，即与单克隆抗体 F(ab')2 片段的结合测定和放射免疫测定的灵敏度进行了比较。在检测单克隆抗独特型抗体时，后者比夹心测定更灵敏，但在分析抗独特型抗血清时灵敏度较低。两种测定方法的灵敏度均低于与单克隆抗体 F(ab')2 片段的结合测定。
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