Department of Microbiology, Karaj Branch, Islamic Azad University, Karaj, Iran.
Department of Biology and Chemistry, Texas A&M International University, 78041, Laredo, TX, USA.
BMC Res Notes. 2023 Oct 12;16(1):268. doi: 10.1186/s13104-023-06544-3.
Polyvinyl alcohol (PVA) as a synthetic biopolymer has unique physicochemical properties to achieve an efficient drug carrier. In this study phenol-substituted polyvinyl alcohol (PVAPh) microparticle was made through a microfluidic system and peroxidase-mediated reaction in the presence of hydrogen peroxide and in following dexamethasone (Dex) release characteristics from this vehicle were elaborated for sustained drug delivery applications.
PVAPh was synthesized by esterification and amidation reactions respectively. Then, the synthesized PVAPh solution containing peroxidase and Dex flowed within the inner channel of the coaxial microfluidic device while liquid paraffin saturated with HO flowed from the outer channel. The monodisperse microparticles were produced in a spherical shape with an average diameter of 160 μm. The Dex was successfully encapsulated in PVAPh MP and its sustained release profile was maintained for up to 7 days. It was found that exposure of Dex-loaded PVAPh MPs to subcultured mouse embryonic fibroblast 10T1/2 cells had no deleterious effects on cell viability, morphology and growth rate. Moreover, the sustained release of Dex and the high mechanical durability of PVAPh MPs suggest an excellent prospect for the synthesized PVAPh and the developed method as a biocompatible carrier required for drug delivery and regenerative medicine.
聚乙烯醇（PVA）作为一种合成生物聚合物，具有独特的物理化学性质，可作为高效的药物载体。本研究通过微流控系统和过氧化物酶介导的反应制备了对苯酚取代的聚乙烯醇（PVAPh）微球，并详细阐述了该载体中过氧化氢存在时的过氧化物酶介导的反应以及随后的地塞米松（Dex）释放特性，以实现药物的持续释放。
通过酯化和酰胺化反应分别合成了 PVAPh。然后，将含有过氧化物酶和 Dex 的合成的 PVAPh 溶液在内通道中流动，而同时液体石蜡在外通道中饱和的 HO 流动。以 160μm 的平均直径产生了单分散的微球。Dex 成功地包封在 PVAPh MPs 中，并保持了长达 7 天的持续释放。结果表明，负载 Dex 的 PVAPh MPs 暴露于亚培养的小鼠胚胎成纤维细胞 10T1/2 细胞中对细胞活力、形态和生长速度没有不良影响。此外，Dex 的持续释放和 PVAPh MPs 的高机械耐久性表明，所合成的 PVAPh 及其开发的方法作为药物输送和再生医学所需的生物相容性载体具有广阔的前景。
