Department of Burn and Plastic Surgery, The First Affiliated Hospital of Soochow University, Suzhou, China.
Department of Burn and Plastic Surgery, Affiliated Hospital of Jiangnan University, Wuxi, China.
J Burn Care Res. 2024 Mar 4;45(2):373-383. doi: 10.1093/jbcr/irad156.
Indocyanine green angiography (ICGA) has been widely employed for quantitative evaluation of the rat comb burn model, but the imaging equipment, imaging protocol, and fluorescence data interpretation of ICGA remain unsatisfactory. This study aims to provide better solutions for the application of ICGA in perfusion analysis. The rat comb burn model was established under a series of different comb contact durations, including 10, 20, 25, 30, 35, and 40 s. Indocyanine green angiography was used to analyze wound perfusion. In total, 16 rats were divided into ibuprofen and control groups for the burn model, and their perfusion was compared. A total of 16 identical models were divided into standard- and high-dose indocyanine green (ICG) groups, and ICGA was conducted to investigate the dynamic change in wound fluorescence. Escharectomy was performed under real-time fluorescence mapping and navigation. The results showed that a comb contact duration of 30 s was optimum for the burn model. Indocyanine green angiography could accurately evaluate the histologically determined depth of thermal injury and wound perfusion in the rat comb model. Digital subtraction of residual fluorescence was necessary for multiple comparisons of perfusion. Dynamic changes in fluorescence and necrotic tissues were observed more clearly by high-dose (0.5 mg/kg) ICG in angiography. In conclusion, perfusion analysis by ICGA can be used to assess the histologically determined depth of thermal injury and the impact of a specific treatment on wound perfusion. Indocyanine green angiography can help to identify necrotic tissue. The above findings and related imaging protocols lay the foundation for future research.
吲哚菁绿血管造影 (ICGA) 已广泛应用于大鼠梳状烧伤模型的定量评估，但 ICGA 的成像设备、成像方案和荧光数据解读仍不尽如人意。本研究旨在为 ICGA 在灌注分析中的应用提供更好的解决方案。在一系列不同的梳子接触时间下建立大鼠梳状烧伤模型，包括 10、20、25、30、35 和 40 s。使用吲哚菁绿血管造影分析伤口灌注。共 16 只大鼠分为布洛芬组和对照组进行烧伤模型，比较其灌注情况。将 16 个相同的模型分为标准和高剂量吲哚菁绿 (ICG) 组，进行 ICGA 以研究伤口荧光的动态变化。实时荧光映射和导航下进行焦痂切除术。结果表明，梳子接触 30 s 是烧伤模型的最佳时间。吲哚菁绿血管造影可以准确评估大鼠梳状模型中热损伤的组织学确定深度和伤口灌注。荧光残留的数字减影对于灌注的多次比较是必要的。在血管造影中，高剂量（0.5 mg/kg）ICG 观察到荧光和坏死组织的动态变化更清晰。总之，ICGA 灌注分析可用于评估热损伤的组织学确定深度和特定治疗对伤口灌注的影响。吲哚菁绿血管造影有助于识别坏死组织。上述发现和相关成像方案为未来的研究奠定了基础。
