The College of Forestry, Shanxi Agricultural University, Jinzhong, Shanxi, China.
Shanxi Key Laboratory of Functional Oil Tree Cultivation and Research, Jinzhong, Shanxi, China.
PLoS One. 2023 Oct 13;18(10):e0292898. doi: 10.1371/journal.pone.0292898. eCollection 2023.
Lipoxygenase (LOX) gene plays an essential role in plant growth, development, and stress response. 15 LOX genes were identified, which were unevenly distributed on chromosomes and divided into three subclasses in this study. In promoter region analysis, many cis-elements were identified in growth and development, abiotic stress response, hormonal response, and light response. qRT-PCR showed that the LOX gene showed tissue specificity in seven tissues, especially XsLOX1, 3, and 7 were relatively highly expressed in roots, stems, and axillary buds. The different expression patterns of LOX genes in response to abiotic stress and hormone treatment indicate that different XsLOX genes have different reactions to these stresses and play diversified roles. This study improves our understanding of the mechanism of LOX regulation in plant growth, development, and stress and lays a foundation for further analysis of biological functions.
脂氧合酶（LOX）基因在植物的生长、发育和应激反应中起着至关重要的作用。本研究共鉴定到 15 个 LOX 基因，它们在染色体上不均匀分布，并分为三个亚类。在启动子区域分析中，发现了许多与生长发育、非生物胁迫响应、激素响应和光响应相关的顺式作用元件。qRT-PCR 结果表明，LOX 基因在 7 种组织中表现出组织特异性，特别是 XsLOX1、3 和 7 在根、茎和腋芽中表达水平相对较高。LOX 基因对非生物胁迫和激素处理的不同表达模式表明，不同的 XsLOX 基因对这些胁迫的反应不同，发挥着多样化的作用。本研究提高了我们对 LOX 在植物生长、发育和应激调节中的机制的理解，为进一步分析其生物学功能奠定了基础。
