Department of Physiology Sciences, State University of Maringá, Maringá, Paraná, Brazil.
Department of Morphology Sciences, State University of Maringá, Maringá, Paraná, Brazil.
Neurogastroenterol Motil. 2023 Dec;35(12):e14688. doi: 10.1111/nmo.14688. Epub 2023 Oct 13.
Our main goals were to investigate the effects of L-glutathione (1%) treatment in Walker-256 tumor-bearing rats by analyzing immunoreactive neurons (IR), responsive to the nNOS enzyme and 3-Nitrotyrosine, in their jejunum myenteric plexus. Moreover, the oxidative state and inflammatory process in these animals were investigated.
Four experimental groups were utilized: control (C), control treated with L-glutathione (CGT), Walker-256 tumor-bearing rats (TW), and Walker-256 tumor-bearing rats treated with L-glutathione (TWGT). After 14 days of tumor inoculation, the jejunum was collected for immunohistochemical techniques and assessment of oxidative status. Plasma was collected to evaluate oxidative status and measure cytokines.
The TW group exhibited a decrease of reduced glutathione in their jejunum, which was prevented in the L-glutathione treated TWGT group. TW animals presented pronounced oxidative stress by increasing levels of lipoperoxidation in their jejunum and malondialdehyde in their plasma; however, the L-glutathione treatment in TWGT group was not able to avoid it. The total antioxidant capacity was altered in groups TW and TWGT, yet the last one had a better index in their plasma. The IL-10, and TNF-α levels increased in TWGT animals. The nNOS-IR neuron density decreased in the jejunum myenteric plexus of the TW group, which was avoided in the TWGT group. The nNOS +3-Nitrotyrosine neurons quantification did not show significative alterations.
The treatment with L-glutathione (1%) imposed an important defense to some parameters of oxidative stress induced by TW-256, leading to neuroprotection to the loss in the nNOS-IR neuron density.
通过分析 Walker-256 荷瘤大鼠空肠肌间神经丛中对 nNOS 酶和 3-硝基酪氨酸有反应的免疫反应性神经元（IR），研究 1%L-谷胱甘肽（L-glutathione）处理对其的影响。此外，还研究了这些动物的氧化状态和炎症过程。
利用 4 个实验组：对照组（C）、对照组用 L-谷胱甘肽处理（CGT）、Walker-256 荷瘤大鼠（TW）、Walker-256 荷瘤大鼠用 L-谷胱甘肽处理（TWGT）。肿瘤接种 14 天后，收集空肠进行免疫组织化学技术和氧化状态评估。收集血浆以评估氧化状态并测量细胞因子。
TW 组空肠组织还原型谷胱甘肽减少，经 L-谷胱甘肽处理的 TWGT 组可预防这种情况。TW 动物的空肠组织脂质过氧化水平和血浆中丙二醛升高，表现出明显的氧化应激；然而，L-谷胱甘肽处理并不能避免这种情况。TW 和 TWGT 组的总抗氧化能力发生了改变，但 TWGT 组的血浆指标更好。TWGT 动物的 IL-10 和 TNF-α 水平升高。TW 组空肠肌间神经丛的 nNOS-IR 神经元密度降低，TWGT 组可避免这种情况。nNOS+3-硝基酪氨酸神经元定量没有明显变化。
1%L-谷胱甘肽（L-glutathione）治疗对 TW-256 诱导的某些氧化应激参数具有重要的防御作用，导致 nNOS-IR 神经元密度降低的神经保护作用。
