非裔美国多发性硬化症（MS）患者的脑脊液中 CD19+和 CD20+B 细胞谱系细胞的比例高于白种人 MS 患者。

African American patients with Multiple Sclerosis (MS) have higher proportions of CD19+ and CD20+ B-cell lineage cells in their cerebrospinal fluid than White MS patients.

机构信息

New York University Grossman School of Medicine, New York, NY, USA.

Department of Pathology, New York University Grossman School of Medicine, New York, NY, USA.

出版信息

Mult Scler Relat Disord. 2023 Nov;79:105047. doi: 10.1016/j.msard.2023.105047. Epub 2023 Oct 2.

DOI:10.1016/j.msard.2023.105047

PMID:37832255

Abstract

OBJECTIVES

To compare proportions of B-cell lineage CD19+ and CD20+ cells in CSF of African-American (AA) and White (W) patients with MS.

BACKGROUND

AA MS patients are more likely to have oligoclonal bands in CSF, higher IgG index in CSF, and higher circulating plasmablasts in blood than W MS patients. It is unknown whether the proportion of B-cells in CSF differs between AA and W patients in MS.

METHODS

Demographics, disease-related information, treatment history were retrospectively collected on patients with MS who self-identified as AA or W and underwent flow cytometry of CSF during diagnostic work-up. Proportion of B-lymphocytes, T-lymphocytes, NK cells, monocytes, and plasma cells were analyzed with flow cytometry.

RESULTS

20 AA and 56 W MS patients fulfilled our inclusion criteria. The groups had similar demographics, CSF cell counts, protein and glucose CSF concentrations, and oligoclonal band number. IgG index was higher in AA compared to W (1.08 vs. 0.85, p = 0.031). AA had higher proportions of CD19+ (5.46 % AA vs. 2.26 % W, p = 0.006) and CD20+ (4.64 % AA vs. 1.91 % W, p = 0.004) cells but did not significantly differ in proportion of CD4+, CD8+, CD38+ bright B-cells, NK cells and monocytes.

CONCLUSIONS

B-cells are overrepresented in the CSF of African American patients with MS relative to Whites.

摘要

目的

比较多发性硬化症（MS）非裔美国（AA）和白人（W）患者脑脊液中 B 细胞谱系 CD19+和 CD20+细胞的比例。

背景

与 W 型 MS 患者相比，AA 型 MS 患者的脑脊液中更可能出现寡克隆带，脑脊液 IgG 指数更高，血液中循环浆母细胞更多。目前尚不清楚 MS 患者的脑脊液中 B 细胞的比例是否在 AA 和 W 之间存在差异。

方法

对自我鉴定为 AA 或 W 并在诊断过程中进行了脑脊液流式细胞术的 MS 患者，回顾性收集人口统计学、与疾病相关的信息和治疗史。通过流式细胞术分析 B 淋巴细胞、T 淋巴细胞、NK 细胞、单核细胞和浆细胞的比例。

结果

20 名 AA 和 56 名 W 型 MS 患者符合我们的纳入标准。两组患者的人口统计学、脑脊液细胞计数、蛋白和葡萄糖 CSF 浓度以及寡克隆带数量相似。与 W 组相比，AA 组 IgG 指数更高（1.08 对 0.85，p=0.031）。AA 组的 CD19+（5.46%AA 对 2.26%W，p=0.006）和 CD20+（4.64%AA 对 1.91%W，p=0.004）细胞比例更高，但 CD4+、CD8+、CD38+bright B 细胞、NK 细胞和单核细胞的比例没有显著差异。