Department of Animal Genetics, Breeding and Reproduction, College of Animal Science and Technology, Henan Agricultural University, China.
Henan Jingwang Pig Breed Improvement Co. LTD, China.
Anim Reprod Sci. 2023 Nov;258:107359. doi: 10.1016/j.anireprosci.2023.107359. Epub 2023 Oct 9.
L-proline has been reported to be useful in semen cryopreservation. However, its use has rarely been reported in the freezing of boar semen. The objective of this study was to evaluate the effects of different concentrations of L-proline (0, 10, 30, 50, and 90 mM) on the quality of boar semen after freezing and thawing. Semen samples from boars (n = 6) were frozen using freezing extenders with added concentrations of L-proline. Total sperm motility, progressive motility, survival time at 37 °C, acrosome integrity, mitochondrial activity, DNA integrity, the content of the lipid peroxidation product malondialdehyde (MDA), total antioxidant capacity (T-AOC) and, expression levels of apoptosis protein (cleaved caspase 3 and Bax) were evaluated after thawing. The results showed that total sperm viability (73.96% vs. 63.58%) and progressive motility (56.88% vs. 47.26%) after thawing were significantly higher in the 10 mM L-proline treatment group than in the control group. The survival time at 37 °C and the total motility of sperm in the 10 mM group within one hour after thawing were significantly higher than in the control group. Acrosome integrity and mitochondrial activity of sperm in the 10 mM group were significantly higher than those in the control, 50 mM, and 90 mM groups. The DNA integrity rate in the 10 mM group was significantly higher than in the control group. The L-proline treatment did not affect sperm MDA content or T-AOC. The expression levels of apoptosis protein (cleaved caspase 3 and Bax) in the 10 mM L-proline supplemented group were lower than those in the control group. In conclusion, the freezing extender containing 10 mM L-proline improved semen quality after freezing and thawing and thus would be a useful reagent for boar semen cryopreservation.
脯氨酸已被报道在精液冷冻保存中有用。然而，它在猪精液冷冻中的应用很少有报道。本研究的目的是评估不同浓度（0、10、30、50 和 90 mM）脯氨酸对猪精液冷冻和解冻后质量的影响。来自公猪（n=6）的精液样本用添加脯氨酸浓度的冷冻稀释液冷冻。在解冻后评估总精子活力、前向运动精子活力、37°C 时的存活时间、顶体完整性、线粒体活性、DNA 完整性、脂质过氧化产物丙二醛（MDA）含量、总抗氧化能力（T-AOC）和凋亡蛋白（裂解 caspase 3 和 Bax）的表达水平。结果表明，与对照组相比，10 mM 脯氨酸处理组解冻后总精子活力（73.96%比 63.58%）和前向运动精子活力（56.88%比 47.26%）显著提高。解冻后 1 小时内，10 mM 组的 37°C 时的存活时间和总精子活力明显高于对照组。10 mM 组精子顶体完整性和线粒体活性明显高于对照组、50 mM 组和 90 mM 组。10 mM 组的 DNA 完整性率明显高于对照组。脯氨酸处理不影响精子 MDA 含量或 T-AOC。添加 10 mM 脯氨酸的凋亡蛋白（裂解 caspase 3 和 Bax）表达水平低于对照组。总之，含有 10 mM 脯氨酸的冷冻稀释液可改善冷冻和解冻后的精液质量，因此可作为猪精液冷冻保存的有用试剂。
