He Mengqian, Sun Lingwei, Xu Jiehuan, Wu Caifeng, Zhang Shushan, Gao Jun, Zhang Defu, Gan Yeqing, Bian Yi, Wei Jinliang, Zhang Weijian, Zhang Wengang, Han Xuejun, Dai Jianjun
Shanghai Municipal Key Laboratory of Agri-Genetics and Breeding, Institute of Animal Husbandry and Veterinary Science, Shanghai Academy of Agricultural Sciences, Shanghai 201106, China.
Key Laboratory of Livestock and Poultry Resources (Pig) Evaluation and Utilization, Ministry of Agriculture and Rural Affairs, Shanghai 201106, China.
Animals (Basel). 2024 May 10;14(10):1422. doi: 10.3390/ani14101422.
To address the safety problems posed by the transportation of boar semen using LN, this study was conducted on the short-term storage of frozen boar semen in dry ice (-79 °C). Boar semen frozen in LN was transferred to dry ice, kept for 1 day, 3 days, 5 days, 7 days, or 8 days, and then moved back to LN. The quality of frozen semen stored in LN or dry ice was determined to evaluate the feasibility of short-distance transportation with dry ice. The results showed that 60 °C for 8 s was the best condition for thawing frozen semen stored in dry ice. No significant differences in spermatozoa motility, plasma membrane integrity, or acrosome integrity were observed in semen after short-term storage in dry ice compared to LN ( > 0.05). There were no significant changes in antioxidant properties between storage groups either ( > 0.05). In conclusion, dry ice could be used as a cold source for the short-term transportation of frozen boar semen for at least 7 days, without affecting sperm motility, morphological integrity, or antioxidant indices.
为解决使用液氮运输公猪精液所带来的安全问题，本研究针对冷冻公猪精液在干冰（-79°C）中的短期储存展开。将液氮中冷冻的公猪精液转移至干冰中，分别保存1天、3天、5天、7天或8天，然后再移回液氮中。通过测定液氮或干冰中储存的冷冻精液质量，以评估使用干冰进行短距离运输的可行性。结果表明，60°C解冻8秒是解冻干冰中储存的冷冻精液的最佳条件。与液氮储存相比，公猪精液在干冰中短期储存后，精子活力、质膜完整性或顶体完整性均未观察到显著差异（P>0.05）。各储存组之间的抗氧化性能也无显著变化（P>0.05）。总之，干冰可作为冷冻公猪精液短期运输的冷源，至少可使用7天，且不影响精子活力、形态完整性或抗氧化指标。
