Yuan Xin, Sun Yiquan, Ni Dong, Xie Zhenwen, Zhang Yanyan, Miao Sun, Wu Linjun, Xing Xin, Zuo Jiane
State Key Joint Laboratory of Environment Simulation and Pollution Control, School of Environment, Tsinghua University, Beijing 100084, China; Research Institute of Highway Ministry of Transport, Beijing 100088, China.
State Key Joint Laboratory of Environment Simulation and Pollution Control, School of Environment, Tsinghua University, Beijing 100084, China.
J Environ Manage. 2023 Dec 15;348:119237. doi: 10.1016/j.jenvman.2023.119237. Epub 2023 Oct 11.
Sulfide produced from sewers is considered one of the dominant threats to public health and sewer lifespan due to its toxicity and corrosiveness. In this study, we developed an environmentally friendly strategy for gaseous sulfide control by enriching indigenous sulfur-oxidizing bacteria (SOB) from sewer sediment. Ceramics acted as bio-carriers for immobilizing SOB for practical use in a lab-scale sewer reactor. 16 S rRNA gene sequences revealed that the SOB consortium was successfully enriched, with Thiobacillus, Pseudomonas, and Alcaligenes occupying a dominant abundance of 64.7% in the microbial community. Metabolic pathway analysis in different acclimatization stages indicates that microorganisms could convert thiosulfate and sulfide into elemental sulfur after enrichment and immobilization. A continuous experiment in lab-scale sewer reactors confirmed an efficient result for sulfide removal with hydrogen sulfide reduction of 43.9% and 85.1% under high-sulfur load and low-sulfur load conditions, respectively. This study shed light on the promising application for sewer sulfide control by biological sulfur oxidation strategy.
下水道产生的硫化物因其毒性和腐蚀性，被认为是对公众健康和下水道使用寿命的主要威胁之一。在本研究中，我们通过从下水道沉积物中富集本地硫氧化细菌（SOB），开发了一种控制气态硫化物的环保策略。陶瓷作为生物载体用于固定SOB，以便在实验室规模的下水道反应器中实际应用。16S rRNA基因序列显示，SOB菌群成功富集，其中硫杆菌属、假单胞菌属和产碱菌属在微生物群落中占主导丰度64.7%。不同驯化阶段的代谢途径分析表明，微生物在富集和固定后可将硫代硫酸盐和硫化物转化为元素硫。实验室规模下水道反应器的连续实验证实了硫化物去除的高效结果，在高硫负荷和低硫负荷条件下，硫化氢去除率分别为43.9%和85.1%。本研究为生物硫氧化策略在下水道硫化物控制中的应用前景提供了启示。
