College of Chemistry and Environmental Engineering, School of Biomedical Engineering, Health Science Center, Shenzhen University, Shenzhen, Guangdong, 518060, PR China; Guangdong Laboratory of Artificial Intelligence and Digital Economy (SZ), Shenzhen, 518060, China; Beijing Key Laboratory for Bioengineering and Sensing Technology, University of Science and Technology Beijing, Beijing, 100083, PR China.
College of Chemistry and Environmental Engineering, School of Biomedical Engineering, Health Science Center, Shenzhen University, Shenzhen, Guangdong, 518060, PR China.
Biosens Bioelectron. 2023 Dec 15;242:115746. doi: 10.1016/j.bios.2023.115746. Epub 2023 Oct 11.
Catalytic hairpin assembly (CHA) is a promising enzyme-free, isothermal signal amplification strategy, but the relatively time-consuming strand replacement limits its application scenarios. Here, we developed an ultrasound-enhanced catalytic hairpin assembly (UECHA) biosensing platform for early screening of Alzheimer's disease by introducing a portable acoustic-drive platform with functionalized microspheres for effective biomarkers enrichment and fluorescence enhancement. By constructing a gradient ultrasonic field in a microcavity, the platform concentrates the functionalized microspheres in a central position, accompanied by an enhanced fluorescence signal with a specific release. In addition, the programmable frequency modulation can also modify the acoustic potential well and effectively promote non-equilibrium chemical reactions such as CHA (25 min). Compared with the conventional catalytic hairpin assembly (CHA), UECHA allows for direct and quantitative measurement of AD miRNAs down to 3.55 × 10 M in 1 μL samples. This visual analysis of ultra-trace biomarkers based on acoustic enrichment and promotion provides a new perspective for the rapid and highly sensitive clinical detection of Alzheimer's disease.
催化发夹组装（CHA）是一种很有前途的无需酶、等温的信号放大策略，但相对耗时的链替换限制了其应用场景。在这里，我们开发了一种超声增强的催化发夹组装（UECHA）生物传感平台，通过引入带有功能化微球的便携式声驱动平台，用于有效富集生物标志物和增强荧光，从而进行阿尔茨海默病的早期筛选。通过在微腔中构建梯度超声场，该平台将功能化微球集中在中央位置，同时伴随着特定释放的增强荧光信号。此外，可编程的频率调制还可以修饰声势能阱，并有效地促进 CHA 等非平衡化学反应（25 分钟）。与传统的催化发夹组装（CHA）相比，UECHA 允许在 1μL 样本中直接、定量地测量 AD miRNAs，下限低至 3.55×10^-17M。这种基于声富集和促进的超痕量生物标志物的可视化分析为阿尔茨海默病的快速、高灵敏度临床检测提供了新视角。
