MOE Key Lab of Rare Pediatric Diseases & Hengyang Medical School, University of South China, Hengyang 421001, China.
Institute for Future Sciences, University of South China, Changsha 410008, China.
Biosensors (Basel). 2024 May 22;14(6):265. doi: 10.3390/bios14060265.
Traditional single nucleic acid assays can only detect one target while multiple nucleic acid assays can detect multiple targets simultaneously, providing comprehensive and accurate information. Fluorescent microspheres in multiplexed nucleic acid detection offer high sensitivity, specificity, multiplexing, flexibility, and scalability advantages, enabling precise, real-time results and supporting clinical diagnosis and research. However, multiplexed assays face challenges like complexity, costs, and sample handling issues. The review explores the recent advancements and applications of fluorescent microspheres in multiple nucleic acid detection. It discusses the versatility of fluorescent microspheres in various fields, such as disease diagnosis, drug screening, and personalized medicine. The review highlights the possibility of adjusting the performance of fluorescent microspheres by modifying concentrations and carrier forms, allowing for tailored applications. It emphasizes the potential of fluorescent microsphere technology in revolutionizing nucleic acid detection and advancing health, disease treatment, and medical research.
传统的单核酸检测只能检测一个靶标，而多核酸检测可以同时检测多个靶标，提供全面和准确的信息。多重核酸检测中的荧光微球具有高灵敏度、特异性、多重性、灵活性和可扩展性优势，能够实现精确、实时的结果，并支持临床诊断和研究。然而，多重检测面临着复杂性、成本和样本处理问题等挑战。本文综述了荧光微球在多重核酸检测中的最新进展和应用。讨论了荧光微球在疾病诊断、药物筛选和个性化医疗等多个领域的多功能性。本文强调了通过改变浓度和载体形式来调整荧光微球性能的可能性，从而实现定制化应用。最后，本文强调了荧光微球技术在改变核酸检测和推进健康、疾病治疗和医学研究方面的潜力。
