Dept of Biological Sciences, Rensselaer Polytechnic Institute, Troy NY, United States.
Dept of Biological Sciences, Rensselaer Polytechnic Institute, Troy NY, United States; Department of Health and Natural Science, Mercy University, Dobbs Ferry, NY, United States.
Vaccine. 2023 Nov 2;41(46):6791-6801. doi: 10.1016/j.vaccine.2023.09.044. Epub 2023 Oct 11.
CatSper is a voltage dependent calcium ion channel present in the principal piece of sperm tail. It plays a crucial role in sperm hyperactivated motility and so in fertilization. Extracellular loops of mouse sperm CatSper were used to develop a vaccine to achieve protection from pregnancy. These loops were inserted at one of the three hypervariable regions of Human Papilloma Virus (HPV) capsid protein (L1). Recombinant vaccines were expressed in E.coli as inclusion body (IB), purified, refolded and assembled into virus-like particles (VLP) in vitro, and adsorbed on alum. Four vaccine candidates were tested in Balb/C mice. All the constructs proved immunogenic, one showed contraceptive efficacy. This recombinant contraceptive vaccine is a non-hormonal intervention and is expected to give long-acting protection from undesired pregnancies.
CatSper 是一种存在于精子尾部主段的电压依赖性钙离子通道。它在精子超激活运动中起着至关重要的作用，因此在受精中也起着至关重要的作用。使用小鼠精子 CatSper 的细胞外环来开发疫苗以实现对妊娠的保护。这些环被插入人乳头瘤病毒 (HPV) 衣壳蛋白 (L1) 的三个高变区之一。重组疫苗在大肠杆菌中作为包涵体 (IB) 表达，然后在体外进行纯化、重折叠并组装成病毒样颗粒 (VLP)，并吸附在明矾上。在 Balb/C 小鼠中测试了四种候选疫苗。所有构建体都被证明具有免疫原性，其中一种具有避孕效果。这种重组避孕疫苗是一种非激素干预措施，有望提供长效避孕保护，防止意外怀孕。
