Núcleo de Pesquisa e Conservação de CervídeosFaculdade de Ciências Agrárias e Veterinárias, Universidade Estadual Paulista, Via de Acesso Prof. Dr. Paulo Donato Castellane, S/N, Jaboticabal, São Paulo, 14884-900, Brazil.
Laboratorio de Reproducción Animal, Facultad de Medicina Veterinaria, Universidad Nacional Mayor de San Marcos, Avenida Circunvalación 28,, San Borja, 15021, Lima, Peru.
Sci Rep. 2023 Oct 13;13(1):17369. doi: 10.1038/s41598-023-43392-4.
The present study aimed to test the efficiency of transcervical artificial insemination techniques with cervical immobilization (TCAI-CI) or cervical traction (TCAI-CT), associated or not with the use of oxytocin (OT) as a protocol for cervical dilation, in the brown brocket deer (Subulo gouazoubira). The study was carried out in a crossover design using four adult females in two replicates with an interval of 60 days. Estrus was synchronized with oral melengestrol acetate (MGA) associated with estradiol benzoate and sodium cloprostenol. TCAI techniques were performed from 18 to 24 h after estrus onset. All females received either an i.v. application of 50 IU of OT (G-OT, n = 4) or 1 mL of saline solution (G-Control, n = 4) 20 min before the TCAI procedure. The TCAIs were performed using frozen-thawed semen motility 40%, vigor 3, acrosome integrity 87%, membrane integrity of 95% and 13% of total post-thaw defects from the same batch. Behavioral estrus was observed in 100% of the females, in both replicates. It was achieved a 50% (4/8) success of cervical transposition with semen deposition in the uterine. Regarding inseminations, most of them (87.5%) were performed using the TCAI-CT technique, and the overall conception rate was 50%. Cervical transposition times (< 1 min) and TCAI procedures (~ 17 min) were considered satisfactory. Thus, the performance of the TCAI-CI and TCAI-CT techniques was successful, regardless of using OT as a cervical dilation protocol. This procedure is proposed as a method of choice for artificial insemination with greater applicability in different conservation centers, compared to more advanced reproductive biotechniques, and with a favorable impact on the conservation of deer species.
本研究旨在测试经颈人工授精技术（TCAI）联合或不联合使用催产素（OT）以实现宫颈扩张，对棕麂（Subulo gouazoubira）的有效性。该研究采用交叉设计，使用 4 只成年雌性动物进行 2 个重复，间隔 60 天。发情通过口服美仑孕酮（MGA）联合苯甲酸雌二醇和氯前列烯醇同步。TCAI 技术在发情开始后 18-24 小时进行。所有雌性动物在 TCAI 程序前 20 分钟，均接受静脉注射 50IU OT（G-OT，n=4）或 1mL 生理盐水溶液（G-Control，n=4）。TCAI 使用同一批精液，活力 3、顶体完整率 87%、膜完整率 95%和总解冻后缺陷 13%的冻融精子进行。在两个重复中，100%的雌性动物观察到性行为发情。在 8 只雌性动物中，有 50%（4/8）成功实现了宫颈转移并将精液注入子宫。关于授精，大多数（87.5%）使用 TCAI-CT 技术进行，总受孕率为 50%。宫颈转移时间（<1 分钟）和 TCAI 程序（~17 分钟）被认为是令人满意的。因此，无论是否使用 OT 作为宫颈扩张方案，TCAI-CI 和 TCAI-CT 技术的性能都是成功的。与更先进的生殖生物技术相比，该程序作为一种更具适用性的人工授精方法，对鹿类物种的保护具有积极影响。
