Arrais Aline Matos, Mello Marco Roberto Bourg de, Vergani Gabriel Brun, Figueira Lucas Machado, Esteves Sérgio Novita, Pereira Verônica Schinaider do Amaral, Bartlewski Pawel Mieczyslaw, Oliveira Maria Emilia Franco, Souza-Fabjan Joanna Maria Gonçalves, Fonseca Jeferson Ferreira da
Departamento de Reprodução e Avaliação Animal, Universidade Federal Rural do Rio de Janeiro, Seropédica, Brazil.
Faculdade de Ciências Agrárias e Veterinárias, Universidade Estadual Paulista, Jaboticabal, Brazil.
Biopreserv Biobank. 2021 Oct;19(5):360-368. doi: 10.1089/bio.2020.0125. Epub 2021 Mar 25.
This study assessed the feasibility of embryo production and nonsurgical embryo recovery (NSER) in Morada Nova ewes (an endangered native Brazilian breed of sheep) subjected to different estrus synchronization and/or superovulation protocols. Ewes received intravaginal sponges soaked with 60 mg medroxyprogesterone acetate (MAP), which were kept in place for six (G6; = 12), nine (G9; = 12), or 12 (G12; = 12) days. Half of the ewes in each group remained estrus synchronized only () and the other half was superovulated () with 133 mg porcine follicle-stimulating hormone (pFSH). There were no differences ( > 0.05) in antral follicle counts determined with ultrasonography 60 hours before MAP sponge removal (or at the time of the first pFSH dose) among G6 (6.4 ± 0.9), G9 (6.2 ± 0.7), and G12 (5.5 ± 0.6). Estrus responses and NSER success rates did not vary ( > 0.05) among the three progestin-treatment groups of ewes for either estrus-induced or superovulated animals. The onset of estrus occurred 10-12 hours later ( < 0.01) in G9 ewes compared with G6 and G12 and the duration of estrus was ∼19 hours greater ( < 0.01) in G9 than in G6. The average duration of the NSER procedure was 32.6 ± 1.3 minutes. At least one structure was recovered in 85.7% of synchronized and in 87.5% of superovulated ewes. Viable embryo recovery rates were also similar ( > 0.05) for G6 (1.0 ± 0.3 and 2.5 ± 1.5), G9 (1.3 ± 0.5 and 4.8 ± 2.0), and G12 groups (1.0 ± 0.3 and 4.8 ± 2.3; estrus-synchronized and superovulated ewes, respectively). In conclusion, progestogen pretreatment of different durations and NSER can be employed in Morada Nova ewes, resulting in reasonable viable embryo recovery rates in both estrus-synchronized and superovulated animals. Therefore, both techniques are suitable for use in commercial settings as well as small ruminant conservation programs.
本研究评估了对莫拉达诺瓦母羊（巴西本土一种濒危绵羊品种）采用不同发情同步和/或超数排卵方案进行胚胎生产及非手术胚胎回收（NSER）的可行性。母羊接受浸泡有60毫克醋酸甲羟孕酮（MAP）的阴道海绵栓，放置6天（G6组；n = 12）、9天（G9组；n = 12）或12天（G12组；n = 12）。每组一半的母羊仅进行发情同步（对照组），另一半用133毫克猪促卵泡素（pFSH）进行超数排卵（处理组）。在取出MAP海绵栓前60小时（或首次注射pFSH时）用超声检查测定的窦卵泡计数，G6组（6.4±0.9）、G9组（6.2±0.7）和G12组（5.5±0.6）之间无差异（P>0.05）。对于诱导发情或超数排卵的母羊，在三个孕激素处理组中，发情反应和NSER成功率没有差异（P>0.05）。与G6组和G12组相比，G9组母羊发情开始时间晚10 - 12小时（P<0.01），且发情持续时间比G6组长约19小时（P<0.01）。NSER程序的平均持续时间为32.6±1.3分钟。在85.7%的同步发情母羊和87.5%的超数排卵母羊中至少回收了一个结构。G6组（分别为1.0±0.3和2.5±1.5）、G9组（1.3±0.5和4.8±2.0）和G12组（1.0±0.3和4.8±2.3；分别为同步发情和超数排卵母羊）的活胚回收率也相似（P>0.05）。总之，不同持续时间的孕激素预处理和NSER可用于莫拉达诺瓦母羊，在同步发情和超数排卵的母羊中均能获得合理的活胚回收率。因此，这两种技术都适用于商业生产以及小型反刍动物保护计划。
