Chen Chujie, Tang Xiangwei, Yan Saina, Yang Anqi, Xiang Jiaojiao, Deng Yanhong, Yin Yulong, Chen Bin, Gu Jingjing
College of Animal Science and Technology, Hunan Agricultural University, Changsha 410128, China.
Hunan Provincial Key Laboratory for Genetic Improvement of Domestic Animal, Hunan Agricultural University, Changsha 410128, China.
Int J Mol Sci. 2023 Sep 23;24(19):14475. doi: 10.3390/ijms241914475.
RNA -methyladenosine (mA) modification is one of the principal post-transcriptional modifications and plays a dynamic role in testicular development and spermatogenesis. However, the role of mA in porcine testis is understudied. Here, we performed a comprehensive analysis of the mA transcriptome-wide profile in Shaziling pig testes at birth, puberty, and maturity. We analyzed the total transcriptome mA profile and found that the mA patterns were highly distinct in terms of the modification of the transcriptomes during porcine testis development. We found that key mA methylated genes (, , , , and ) were highly enriched during spermatogenesis and identified in spermatogenesis-related KEGG pathways, including Wnt, cAMP, mTOR, AMPK, PI3K-Akt, and spliceosome. Our findings indicated that mA methylations are involved in the complex yet well-organized post-transcriptional regulation of porcine testicular development and spermatogenesis. We found that the mA eraser negatively regulated the proliferation of immature porcine Sertoli cells. Furthermore, we proposed a novel mechanism of mA modification during testicular development: regulated the RNA methylation level and gene expression of mRNA. In addition to serving as a potential target for improving boar reproduction, our findings contributed to the further understanding of the regulation of mA modifications in male reproduction.
RNA甲基腺苷（mA）修饰是主要的转录后修饰之一，在睾丸发育和精子发生过程中发挥着动态作用。然而，mA在猪睾丸中的作用尚未得到充分研究。在此，我们对沙子岭猪出生、青春期和成熟期睾丸的全转录组mA图谱进行了全面分析。我们分析了全转录组的mA图谱，发现猪睾丸发育过程中转录组修饰方面的mA模式存在高度差异。我们发现关键的mA甲基化基因（……此处原文关键基因罗列部分缺失，无法准确补充……）在精子发生过程中高度富集，并在与精子发生相关的KEGG通路中被鉴定出来，包括Wnt、cAMP、mTOR、AMPK、PI3K - Akt和剪接体。我们的研究结果表明，mA甲基化参与了猪睾丸发育和精子发生复杂而有序的转录后调控。我们发现mA去甲基化酶负向调节未成熟猪支持细胞的增殖。此外，我们提出了睾丸发育过程中mA修饰的一种新机制：……此处原文相关分子罗列部分缺失，无法准确补充……调节了……此处原文mRNA相关内容缺失，无法准确补充……的RNA甲基化水平和基因表达。除了作为改善公猪繁殖的潜在靶点外，我们的研究结果有助于进一步了解男性生殖中mA修饰的调控。
