Department of Pharmacy-Pharmaceutical Sciences, University of Bari Aldo Moro, Via E. Orabona 4, 70125 Bari, Italy.
Department of Pharmacy, University of Naples Federico II, Via D. Montesano 49, 80131 Naples, Italy.
Int J Mol Sci. 2023 Sep 25;24(19):14523. doi: 10.3390/ijms241914523.
This study aimed to examine the impact of different surface properties of poly(lactic-co-glycolic) acid (PLGA) nanoparticles (P NPs) and PLGA-Poloxamer nanoparticles (PP NPs) on their in vivo biodistribution. For this purpose, NPs were formulated via nanoprecipitation and loaded with diphenylhexatriene (DPH), a fluorescent dye. The obtained NPs underwent comprehensive characterization, encompassing their morphology, technological attributes, DPH release rate, and thermodynamic properties. The produced NPs were then administered to wild-type mice via intraperitoneal injection, and, at scheduled time intervals, the animals were euthanized. Blood samples, as well as the liver, lungs, and kidneys, were extracted for histological examination and biodistribution analysis. The findings of this investigation revealed that the presence of poloxamers led to smaller NP sizes and induced partial crystallinity in the NPs. The biodistribution and histological results from in vivo experiments evidenced that both, P and PP NPs, exhibited comparable concentrations in the bloodstream, while P NPs could not be detected in the other organs examined. Conversely, PP NPs were primarily sequestered by the lungs and, to a lesser extent, by the kidneys. Future research endeavors will focus on investigating the behavior of drug-loaded NPs in pathological animal models.
本研究旨在探讨聚（乳酸-共-乙醇酸）（PLGA）纳米粒子（P NPs）和 PLGA-泊洛沙姆纳米粒子（PP NPs）的不同表面性质对其体内分布的影响。为此，通过纳米沉淀法制备了负载二苯基己三烯（DPH）的荧光染料的 NPs。所得 NPs 进行了全面的表征，包括形态、技术特性、DPH 释放率和热力学性质。然后将制备的 NPs 通过腹腔注射给予野生型小鼠，并在预定的时间间隔处死动物。提取血液样本以及肝脏、肺和肾脏进行组织学检查和生物分布分析。研究结果表明，泊洛沙姆的存在导致 NP 尺寸变小，并使 NPs 产生部分结晶。体内实验的生物分布和组织学结果表明，P 和 PP NPs 在血液中的浓度相当，而 P NPs 无法在其他检查的器官中检测到。相反，PP NPs 主要被肺和肾脏摄取，程度较轻。未来的研究将集中于研究载药 NP 在病理性动物模型中的行为。
