Zhejiang Provincial Key Laboratory of Biometrology and Inspection & Quarantine, College of Life Sciences, China Jiliang University, Hangzhou 310018, China.
Int J Mol Sci. 2023 Oct 8;24(19):14987. doi: 10.3390/ijms241914987.
The recent pandemic of SARS-CoV-2 has underscored the critical need for rapid and precise viral detection technologies. Point-of-care (POC) technologies, which offer immediate and accurate testing at or near the site of patient care, have become a cornerstone of modern medicine. Prokaryotic Argonaute proteins (pAgo), proficient in recognizing target RNA or DNA with complementary sequences, have emerged as potential game-changers. pAgo present several advantages over the currently popular CRISPR/Cas systems-based POC diagnostics, including the absence of a PAM sequence requirement, the use of shorter nucleic acid molecules as guides, and a smaller protein size. This review provides a comprehensive overview of pAgo protein detection platforms and critically assesses their potential in the field of viral POC diagnostics. The objective is to catalyze further research and innovation in pAgo nucleic acid detection and diagnostics, ultimately facilitating the creation of enhanced diagnostic tools for clinic viral infections in POC settings.
最近的 SARS-CoV-2 大流行凸显了快速、准确的病毒检测技术的重要性。即时检测（POC）技术可在患者护理现场或附近提供即时、准确的检测，已成为现代医学的基石。原核 Argonaute 蛋白（pAgo）在识别具有互补序列的靶 RNA 或 DNA 方面表现出色，已成为潜在的变革者。与目前流行的基于 CRISPR/Cas 系统的 POC 诊断相比，pAgo 具有几个优势，包括不需要 PAM 序列、使用较短的核酸分子作为向导以及蛋白质尺寸更小。本综述全面概述了 pAgo 蛋白检测平台，并批判性地评估了它们在病毒 POC 诊断领域的潜力。目的是促进 pAgo 核酸检测和诊断的进一步研究和创新，最终为 POC 环境中的临床病毒感染创建增强的诊断工具。
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