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磷酸化鱼明胶与果冻凝胶的品质:凝胶化及微生物组学分析

Phosphorylated Fish Gelatin and the Quality of Jelly Gels: Gelling and Microbiomics Analysis.

作者信息

Wu Shiyu, Sun Wanyi, Yang Yihui, Jia Ru, Zhan Shengnan, Ou Changrong, Huang Tao

机构信息

College of Food and Pharmaceutical Science, Ningbo University, Ningbo 315211, China.

Key Laboratory of Animal Protein Food Deep Processing Technology of Zhejiang Province, Ningbo University, Ningbo 315832, China.

出版信息

Foods. 2023 Oct 7;12(19):3682. doi: 10.3390/foods12193682.

Abstract

Phosphorylated fish gelatin (PFG) exhibited preferable physical and chemical properties than fish gelatin (FG) in our previous study. To investigate the application values of PFG, the effects of different ratios (2:1, 1:1 and 1:2) of FG(PFG)/κ carrageenan (κC) on the quality of jelly gels (JGs) were investigated. The sensory quality of PFG:κC (1:2)/FG:κC (1:2) was found to be superior based on sensory evaluations, which was also verified with the results for texture, rheology, etc. Moreover, the structural changes in JGs were related to the introduction of phosphoric acid groups into the molecular chain of gelatin and the protein-polysaccharide interactions. According to the storage results, PFG jelly had better storage quality, higher hardness and chewiness values than those of FG jelly. High-throughput sequencing of JG microbial analysis showed that the addition of PFG changed the amount of microorganisms, microbial species abundance and the microbial composition of JGs, which were also closely related to the storage quality of JGs. In conclusion, the applications of PFG have promising potential to improve the quality of confectionery.

摘要

在我们之前的研究中,磷酸化鱼明胶(PFG)表现出比鱼明胶(FG)更优的物理和化学性质。为了研究PFG的应用价值,我们研究了不同比例(2:1、1:1和1:2)的FG(PFG)/κ-卡拉胶(κC)对果冻凝胶(JGs)品质的影响。基于感官评价发现PFG:κC(1:2)/FG:κC(1:2)的感官品质更优,这也通过质地、流变学等结果得到了验证。此外,JGs的结构变化与磷酸基团引入明胶分子链以及蛋白质-多糖相互作用有关。根据储存结果,PFG果冻比FG果冻具有更好的储存品质、更高的硬度和咀嚼度值。JG微生物分析的高通量测序表明,PFG的添加改变了JGs中的微生物数量、微生物种类丰度和微生物组成,这也与JGs的储存品质密切相关。总之,PFG的应用在改善糖果品质方面具有广阔的潜力。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6d97/10572387/cd123723c17a/foods-12-03682-g001.jpg

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