College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, P. R. China.
Key Lab of Animal Physiology and Biochemistry, Ministry of Agriculture, Nanjing, P. R. China.
J Microbiol. 2017 Sep;55(9):694-702. doi: 10.1007/s12275-017-6627-9. Epub 2017 Sep 2.
In China's swine industry, copper is generally supplemented above the National Research Council (NRC) requirement (2012) because of its antimicrobial properties and the potential for growth promotion. Yet few are concerned about whether this excess supplementation is necessary. In this study, the 16S rRNA pyrosequencing was designed and used to investigate the effect of dietary copper level on the diversity of the fecal microbial community and the correlation of copper level with the serum level of inflammatory cytokines in Sprague-Dawley rat models. The results showed that the diet containing a high level of Cu (120 and 240 mg/kg) changed the microbial richness and diversity of rat feces associated with the increased copper content in the rat ileac and colonic digesta. Furthermore, a Pearson's correlation analysis indicated that an accumulation of unabsorbed copper in the chyme was correlated with the microbial composition of the rat feces, which was linked with TNF-α in serum. The results suggest that dietary copper level may have a direct impact on circulating inflammatory cytokines in the serum, perhaps inducing an inflammatory response by altering the microbial composition of rat feces. Serum TNF-α could be the chief responder to excessive copper exposure.
在中国的养猪业中，由于铜具有抗菌性能和促进生长的潜力，通常会在国家研究委员会（NRC）的要求（2012 年）之上补充铜。然而，很少有人关注这种过量补充是否有必要。在这项研究中，设计并使用了 16S rRNA 焦磷酸测序来研究日粮铜水平对粪便微生物群落多样性的影响，以及铜水平与 Sprague-Dawley 大鼠模型血清中炎症细胞因子水平的相关性。结果表明，含有高铜（120 和 240mg/kg）的饮食改变了大鼠粪便的微生物丰富度和多样性，这与大鼠回肠和结肠内容物中铜含量的增加有关。此外，皮尔逊相关性分析表明，食糜中未被吸收的铜的积累与大鼠粪便的微生物组成有关，这与血清中的 TNF-α有关。研究结果表明，日粮铜水平可能直接影响血清中的循环炎症细胞因子，可能通过改变大鼠粪便的微生物组成引起炎症反应。血清 TNF-α可能是对过量铜暴露的主要反应者。
