Kiewisz Jolanta, Waśniewski Tomasz, Kieżun Jacek, Skowrońska Agnieszka, Kaczmarek Monika M, Szóstak Błażej, Kowalczyk Anna E, Kmieć Zbigniew
Department of Human Histology and Embryology, School of Medicine, Collegium Medicum, University of Warmia and Mazury, 10-082 Olsztyn, Poland.
Department of Gynecology and Obstetrics, School of Medicine, Collegium Medicum, University of Warmia and Mazury, 10-082 Olsztyn, Poland.
Cancers (Basel). 2023 Sep 28;15(19):4780. doi: 10.3390/cancers15194780.
The inappropriate action of WNT4 and estrogens affects uterine homeostasis and function, and may lead to endometrial cancer (EC).
The aim was to evaluate the alterations of gene expression and WNT4 protein immunoreactivity (Ir) in EC, considering tumor characteristics, the clinicopathological association and estrogen dependence.
mRNA levels were compared between benign (control) endometrium ( = 8) and endometroid EC (EEC) and non-endometroid EC (non-EEC) samples ( = 28) using the real-time PCR technique. The WNT4-Ir and ERα-Ir were evaluated by immunohistochemistry (IHC). mRNA gene and WNT4-Ir were correlated with clinicopathological and blood morphological parameters. Overall survival (OS) was assessed. The bioanalysis was utilized to study WNT4 expression in large patient cohort ( = 549).
gene expression was decreased in EC samples (specifically in EEC but not in non-EEC) compared to the control. The gene expression was also decreased in EC samples categorized by the tumor characteristics. There was no statistical difference in WNT4-Ir or ERα-Ir between the control and EC. There was no correlation between OS and gene expression and WNT4-Ir. Bioanalysis showed that and gene expression alterations tended to be mutually exclusive. An alteration in expression was found in different histological tumor types in a large group of EC patients.
There is a great need to evaluate the molecular background of EC. Our study suggests that the gene has the potential to be a marker of functional estrogen signaling in EEC.
WNT4和雌激素的不适当作用会影响子宫内环境稳定和功能，并可能导致子宫内膜癌（EC）。
旨在评估EC中基因表达和WNT4蛋白免疫反应性（Ir）的改变，同时考虑肿瘤特征、临床病理关联和雌激素依赖性。
使用实时PCR技术比较良性（对照）子宫内膜（n = 8）与子宫内膜样EC（EEC）和非子宫内膜样EC（非EEC）样本（n = 28）之间的mRNA水平。通过免疫组织化学（IHC）评估WNT4-Ir和ERα-Ir。将mRNA基因和WNT4-Ir与临床病理和血液形态学参数进行关联分析。评估总生存期（OS）。利用生物分析研究一大组患者队列（n = 549）中WNT4的表达情况。
与对照相比，EC样本中基因表达降低（特别是在EEC中而非非EEC中）。根据肿瘤特征分类的EC样本中基因表达也降低。对照与EC之间的WNT4-Ir或ERα-Ir无统计学差异。OS与基因表达和WNT4-Ir之间无相关性。生物分析表明，和基因表达改变倾向于相互排斥。在一大组EC患者的不同组织学肿瘤类型中发现了表达改变。
非常有必要评估EC的分子背景。我们的研究表明，基因有可能成为EEC中功能性雌激素信号的标志物。
