Zou Shuzhen, Yuan Tingting, Lu Tan, Yan Jiayu, Kang Di, Li Dayong
Key Laboratory of Southwest China Wildlife Resources Conservation of Ministry of Education, China West Normal University, 1# Shida Road, Nanchong 637009, China.
Key Laboratory of Conservation Biology of Rhinopithecus Roxellana at China West Normal University of Sichuan Province, China West Normal University, 1# Shida Road, Nanchong 637009, China.
Animals (Basel). 2023 Oct 3;13(19):3083. doi: 10.3390/ani13193083.
From the perspective of interactions in the human-animal-ecosystem, the study and control of pathogenic bacteria that can cause disease in animals and humans is the core content of "One Health". In order to test the effect of human disturbance (HD) on the health risk of pathogenic antibiotic-resistant bacteria (PARBs) to wild animals and transfer risk of the PARBs from wild animals to humans, golden snub-nosed monkeys () were used as sentinel animals. Metagenomic analysis was used to analyze the characteristics of PARBs in the gut microbiota of golden snub-nosed monkeys. Then, the total contribution of antibiotic resistance genes (ARGs) and virulence factors (VFs) of the PARBs were used to assess the health risk of PARBs to golden snub-nosed monkeys, and the antimicrobial drug resistance and bacterial infectious disease of PARBs were determined to assess the transfer risk of PARBs from golden snub-nosed monkeys to humans. There were 18 and 5 kinds of PARBs in the gut microbiota of golden snub-nosed monkeys under HD (HD group) and wild habitat environments (W group), respectively. The total health risks of PARBs to the W group and the HD group were -28.5 × 10 and 125.8 × 10, respectively. There were 12 and 16 kinds of KEGG pathways of human diseases in the PARBs of the W group and the HD group, respectively, and the gene numbers of KEGG pathways in the HD group were higher than those in the W group. HD increased the pathogenicity of PARBs to golden snub-nosed monkeys, and the PARBs in golden snub-nosed monkeys exhibited resistance to lincosamide, aminoglycoside, and streptogramin antibiotics. If these PARBs transfer from golden snub-nosed monkeys to humans, then humans may acquire symptoms of pathogens including , , , , , and .
从人-动物-生态系统相互作用的角度来看，对可在动物和人类中致病的病原菌的研究与控制是“同一健康”的核心内容。为了测试人类干扰（HD）对野生抗生素抗性病原菌（PARBs）给野生动物带来的健康风险以及PARBs从野生动物向人类的传播风险，川金丝猴被用作哨兵动物。采用宏基因组分析来分析川金丝猴肠道微生物群中PARBs的特征。然后，利用PARBs的抗生素抗性基因（ARGs）和毒力因子（VFs）的总贡献来评估PARBs对川金丝猴的健康风险，并确定PARBs的抗菌药物耐药性和细菌传染病，以评估PARBs从川金丝猴向人类的传播风险。在HD环境下（HD组）和野生栖息地环境（W组）的川金丝猴肠道微生物群中，分别有18种和5种PARBs。PARBs对W组和HD组的总健康风险分别为-28.5×10和125.8×10。W组和HD组的PARBs中分别有12种和16种人类疾病的KEGG通路，且HD组中KEGG通路的基因数量高于W组。HD增加了PARBs对川金丝猴的致病性，川金丝猴体内的PARBs对林可酰胺类、氨基糖苷类和链阳菌素类抗生素表现出抗性。如果这些PARBs从川金丝猴传播到人类，那么人类可能会出现包括[此处原文缺失具体病症]等病原体的症状。
