Zhou Fangting, Fan Xinyang, Xu Xiaoqi, Li Zhuoran, Qiu Lihua, Miao Yongwang
Faculty of Animal Science and Technology, Yunnan Agricultural University, Kunming 650201, China.
College of Chemistry, Biology and Environment, Yuxi Normal University, Yuxi 653100, China.
Animals (Basel). 2023 Oct 9;13(19):3156. doi: 10.3390/ani13193156.
The ATP-binding cassette subfamily G member 2 () serves crucial roles in secreting riboflavin and biotin vitamins into the milk of cattle, mice, and humans, as well as in the transportation of xenotoxic and cytostatic drugs across the plasma membrane. However, the specific role of the gene in water buffaloes (), especially its effect on milk fat synthesis in buffalo mammary epithelial cells (BuMECs), remains inadequately understood. In this study, the full-length CDS of the buffalo gene was isolated and identified from the mammary gland in buffaloes. A bioinformatics analysis showed a high degree of similarity in the transcriptional region, motifs, and conservative domains of the buffalo with those observed in other Bovidae species. The functional role of buffalo was associated with the transportation of solutes across lipid bilayers within cell membranes. Among the 11 buffalo tissues detected, the expression levels of were the highest in the liver and brain, followed by the mammary gland, adipose tissue, heart, and kidney. Notably, its expression in the mammary gland was significantly higher during peak lactation than during non-lactation. The gene was identified with five SNPs in river buffaloes, while it was monomorphic in swamp buffaloes. Functional experiments revealed that increased the triglyceride (TAG) content by affecting the expression of liposynthesis-related genes in BuMECs. The results of this study underscore the pivotal role of the gene in influencing the milk fat synthesis in BuMECs.
ATP结合盒亚家族G成员2（）在将核黄素和生物素维生素分泌到牛、小鼠和人类的乳汁中，以及在将异源毒性和细胞抑制药物跨质膜转运方面发挥着关键作用。然而，该基因在水牛（）中的具体作用，尤其是其对水牛乳腺上皮细胞（BuMECs）中乳脂肪合成的影响，仍未得到充分了解。在本研究中，从水牛的乳腺中分离并鉴定了水牛基因的全长CDS。生物信息学分析表明，水牛的转录区域、基序和保守结构域与其他牛科物种中观察到的高度相似。水牛的功能作用与溶质跨细胞膜脂质双层的转运有关。在检测的11种水牛组织中，在肝脏和大脑中的表达水平最高，其次是乳腺、脂肪组织、心脏和肾脏。值得注意的是，其在乳腺中的表达在泌乳高峰期明显高于非泌乳期。该基因在河流型水牛中鉴定出5个单核苷酸多态性（SNP），而在沼泽型水牛中为单态性。功能实验表明，通过影响BuMECs中脂肪合成相关基因的表达增加了甘油三酯（TAG）含量。本研究结果强调了该基因在影响BuMECs中乳脂肪合成方面的关键作用。
