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下一代测序与基因组编辑时代的诱变

Mutagenesis in the Age of Next-Generation-Sequencing and Genome Editing.

作者信息

Xin Zhanguo

机构信息

Plant Stress and Germplasm Development Unit, USDA-ARS, 3810 4th Street, Lubbock, TX 79415, USA.

出版信息

Plants (Basel). 2023 Sep 27;12(19):3403. doi: 10.3390/plants12193403.

DOI:10.3390/plants12193403
PMID:37836142
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10574159/
Abstract

Mutagenesis is a proven, classical technique for inducing a broad spectrum of DNA variations and has led to the creation of thousands of improved varieties in many crop species [...].

摘要

诱变是一种成熟的经典技术，可诱导产生广泛的DNA变异，并已在许多作物品种中培育出了数千个改良品种[……]。

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本文引用的文献

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A large sequenced mutant library - valuable reverse genetic resource that covers 98% of sorghum genes.一个大型的测序突变体文库——宝贵的反向遗传学资源，涵盖了高粱 98%的基因。
Plant J. 2024 Mar;117(5):1543-1557. doi: 10.1111/tpj.16582. Epub 2023 Dec 15.
2
Variations in Total Protein and Amino Acids in the Sequenced Sorghum Mutant Library.测序高粱突变体文库中总蛋白和氨基酸的变异
Plants (Basel). 2023 Apr 15;12(8):1662. doi: 10.3390/plants12081662.
3
Generation of Sesame Mutant Population by Mutagenesis and Identification of High Oleate Mutants by GC Analysis.通过诱变产生芝麻突变群体并利用气相色谱分析鉴定高油酸突变体
Plants (Basel). 2023 Mar 13;12(6):1294. doi: 10.3390/plants12061294.
4
Large-scale genome editing in plants: approaches, applications, and future perspectives.植物的大规模基因组编辑：方法、应用和未来展望。
Curr Opin Biotechnol. 2023 Feb;79:102875. doi: 10.1016/j.copbio.2022.102875. Epub 2023 Jan 5.
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Genome Survey Sequencing and Genetic Background Characterization of Sims (Aquifoliaceae) Based on Next-Generation Sequencing.基于二代测序的冬青属植物基因组Survey测序及遗传背景特征分析
Plants (Basel). 2022 Dec 1;11(23):3322. doi: 10.3390/plants11233322.
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Spectrum and Density of Gamma and X-ray Induced Mutations in a Non-Model Rice Cultivar.非模式水稻品种中γ射线和X射线诱导突变的谱与密度
Plants (Basel). 2022 Nov 25;11(23):3232. doi: 10.3390/plants11233232.
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Soybean Protein and Oil Variants Identified through a Forward Genetic Screen for Seed Composition.通过正向遗传学筛选种子成分鉴定出的大豆蛋白和油变体
Plants (Basel). 2022 Nov 3;11(21):2966. doi: 10.3390/plants11212966.
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Targeted Mutagenesis of the Multicopy Gene Family in Allotetraploid Reduces Pungency in Fresh Leaves across Environments.异源四倍体中多拷贝基因家族的靶向诱变降低了不同环境下新鲜叶片的辛辣味。
Plants (Basel). 2022 Sep 23;11(19):2494. doi: 10.3390/plants11192494.
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Plants (Basel). 2022 Jul 4;11(13):1772. doi: 10.3390/plants11131772.
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Targeting TOR and SnRK1 Genes in Rice with CRISPR/Cas9.利用CRISPR/Cas9靶向水稻中的TOR和SnRK1基因
Plants (Basel). 2022 May 30;11(11):1453. doi: 10.3390/plants11111453.