Chazin W J, Wüthrich K, Hyberts S, Rance M, Denny W A, Leupin W
J Mol Biol. 1986 Aug 5;190(3):439-53. doi: 10.1016/0022-2836(86)90014-8.
Sequence-specific 1H nuclear magnetic resonance assignments are presented for d-(GCATTAATGC)2. Using omega 1-scaled double quantum-filtered correlated spectroscopy, two-quantum spectroscopy, relayed coherence transfer spectroscopy and detailed analysis of the fine structure in these phase-sensitive spectra, the spin system of the bases and deoxyribose rings were identified entirely via scalar proton-proton couplings. The sequential connectivities were established with two-dimensional nuclear Overhauser enhancement spectra recorded with a short mixing time of 60 milliseconds. These spectra contain only a small number of cross-peaks, corresponding to the shortest proton-proton distances prevailing in the DNA. They are thus easy to interpret, and therefore the presently proposed modifications of the established assignment procedures should enable studies of larger DNA duplexes with intrinsically more complex nuclear magnetic resonance spectra, and they also provided an improved basis for conformational studies of DNA fragments.
给出了d-(GCATTAATGC)2的序列特异性1H核磁共振归属。使用ω1标度双量子滤波相关光谱、双量子光谱、接力相干转移光谱以及对这些相敏光谱精细结构的详细分析，通过标量质子-质子耦合完全确定了碱基和脱氧核糖环的自旋系统。利用混合时间为60毫秒的短混合时间记录的二维核Overhauser增强光谱建立了序列连接性。这些光谱仅包含少量交叉峰，对应于DNA中存在的最短质子-质子距离。因此它们易于解释，因此目前对既定归属程序的改进应能使对具有本质上更复杂核磁共振光谱的更大DNA双链体进行研究，并且它们还为DNA片段的构象研究提供了更好的基础。
