Department of Zoology, Faculty of Science, Zagazig University, Zagazig, Egypt.
Department of Pharmacognosy, Faculty of Pharmacy, Zagazig University, Zagazig, Egypt.
Naunyn Schmiedebergs Arch Pharmacol. 2024 Apr;397(4):2389-2400. doi: 10.1007/s00210-023-02737-6. Epub 2023 Oct 14.
7,12-Dimethylbenzanthracene (DMBA) is a member of the polycyclic aromatic hydrocarbon family. It is a member of the polycyclic aromatic hydrocarbon family. It is a mutagenic, carcinogenic, and immunosuppressor agent. Cannabidiol (CBD) is a phytocannabinoid. It has anticonvulsant, anti-inflammatory, anti-anxiety, antioxidant, and anti-cancer properties. The purpose of this study was to investigate the possible protective and therapeutic benefits of CBD oil in DMBA-induced leukemia in rats.
Experimental animals were divided into six groups of five rats each. Group 1 (normal control) included healthy rats. Group 2 included normal rats that received olive oil. Group 3 included normal rats that received CBD. Group 4 included the DMBA-induced leukemic group. Group 5 (prophylactic group) included rats that received CBD as a prophylaxis before IV injection with DMBA. Group 6 (treated group) included DMBA-induced leukemic rats that received CBD as treatment. Liver functions (total, direct and indirect bilirubin, alkaline phosphatase (ALP), alanine transaminase (ALT), aspartate aminotransferase (AST), albumin, globulin, and albumin globulin ratio) were measured. Superoxide dismutase (SOD) and catalase (CAT) were also measured. Total RNA extraction followed by-real time qRT-PCR gene expression of LC3-II, Beclin, mTOR, and P62 was performed. Histopathological examination of liver and spleen tissues was performed.
Administration of CBD in groups 5 and 6 resulted in a significant improvement of the levels of liver functions compared to the leukemic untreated rats. Also, the levels of catalase and SOD significantly increased after treatment with CBD compared to the leukemic group. After treatment with CBD in groups 5 and 6, there were downregulations in the expression of all studied genes compared to leukemic untreated rats. Treatment with CBD was more statistically effective than prophylactic use.
Administration of CBD resulted in a significant improvement in the biochemical, antioxidant status, morphological, and molecular measures in DMBA-induced leukemia in adult male rats. The therapeutic use was more effective than the prophylactic one.
7,12-二甲基苯并蒽（DMBA）是多环芳烃家族的一员。它是一种致突变、致癌和免疫抑制剂。大麻二酚（CBD）是一种植物大麻素。它具有抗惊厥、抗炎、抗焦虑、抗氧化和抗癌特性。本研究旨在探讨 CBD 油对 DMBA 诱导的大鼠白血病的可能保护和治疗作用。
实验动物分为五组，每组五只。第 1 组（正常对照组）包括健康大鼠。第 2 组包括接受橄榄油的正常大鼠。第 3 组包括接受 CBD 的正常大鼠。第 4 组包括 DMBA 诱导的白血病组。第 5 组（预防组）包括在 IV 注射 DMBA 前接受 CBD 预防的大鼠。第 6 组（治疗组）包括接受 CBD 治疗的 DMBA 诱导的白血病大鼠。测量肝功能（总胆红素、直接胆红素、间接胆红素、碱性磷酸酶（ALP）、丙氨酸转氨酶（ALT）、天冬氨酸转氨酶（AST）、白蛋白、球蛋白和白蛋白球蛋白比值）。还测量了超氧化物歧化酶（SOD）和过氧化氢酶（CAT）。进行总 RNA 提取，然后进行实时 qRT-PCR 基因表达分析，分析 LC3-II、Beclin、mTOR 和 P62。对肝和脾组织进行组织病理学检查。
与未治疗的白血病大鼠相比，第 5 组和第 6 组 CBD 给药导致肝功能水平显著改善。与白血病组相比，CBD 治疗后 CAT 和 SOD 水平显著升高。与未治疗的白血病大鼠相比，第 5 组和第 6 组 CBD 治疗后所有研究基因的表达均下调。与预防使用相比，CBD 治疗更具统计学意义。
在成年雄性大鼠的 DMBA 诱导的白血病中，CBD 的给药导致生化、抗氧化状态、形态和分子测量显著改善。治疗用途比预防用途更有效。
