Ajiboye B O, Dada S, Fatoba H O, Lawal O E, Oyeniran O H, Adetuyi O Y, Olatunde A, Taher M, Khotib J, Susanti D, Oyinloye B E
Phytomedicine and Molecular Toxicology Research Laboratory, Department of Biochemistry, Federal University Oye-Ekiti, Ekiti State, Nigeria; Institute of Drug Research and Development, SE Bogoro Center, Afe Babalola University, Ado-Ekiti, Nigeria.
Phytomedicine, Biochemical Toxicology and Biotechnology Research Laboratories, Department of Biochemistry, College of Sciences, Afe Babalola University, Ado-Ekiti, Nigeria.
Biomed Pharmacother. 2023 Dec;168:115681. doi: 10.1016/j.biopha.2023.115681. Epub 2023 Oct 12.
This experiment was conducted to evaluate the Dalbergiella welwitschia alkaloid-rich extracts on liver damage in streptozotocin-induced diabetic rats. Hence, to induce diabetes, 45 mg/kg body weight of streptozotocin was intraperitoneally injected into the Wistar rats. Subsequently, 5 % (w/v) of glucose water was given to the induced animals for 24 h. Thus, the animals (48) were grouped into five groups (n = 8), containing normal control (NC), diabetic control (DC), diabetic rats placed on low (50 mg/kg body weight) and high (100 mg/kg body weight) doses of D. welwitschi alkaloid-rich leaf extracts (i.e. DWL and DWH respectively), and diabetic rats administered 200 mg/kg body weight of metformin (MET). The animals were sacrificed on the 21st day of the experiment, blood and liver were harvested, and different liver damage biomarkers were evaluated. The results obtained demonstrated that diabetic rats administered DWL, DWH and MET significantly (p < 0.05) increased hepatic AST, ALT, albumin, SOD, CAT, GSH, and GPX levels when compared to DC with no significant (p > 0.05) different when compared with NC. Also, diabetic rats administered DWL, DWH and MET revealed a significant (p < 0.05) decrease in GGT and MDA levels, as well as, fragmented DNA and protein carbonyl levels when compared to DC with no significant (p > 0.05) different when compared with NC. In addition, histological examination revealed that diabetic rats placed on DWL, DWH and MET normalized the hepatocytes. Consequently, it can be inferred that alkaloid-rich extracts from D. welwitschi leaf could be helpful in improving liver damage associated with diabetes mellitus rats.
本实验旨在评估富含威尔威茨氏黄檀生物碱的提取物对链脲佐菌素诱导的糖尿病大鼠肝脏损伤的影响。因此，为诱导糖尿病，将45mg/kg体重的链脲佐菌素腹腔注射到Wistar大鼠体内。随后，给诱导的动物提供5%（w/v）的葡萄糖水24小时。因此，将48只动物分为五组（n = 8），包括正常对照组（NC）、糖尿病对照组（DC）、给予低剂量（50mg/kg体重）和高剂量（100mg/kg体重）富含威尔威茨氏黄檀生物碱叶提取物的糖尿病大鼠（分别为DWL和DWH），以及给予200mg/kg体重二甲双胍（MET）的糖尿病大鼠。在实验的第21天处死动物，采集血液和肝脏，并评估不同的肝脏损伤生物标志物。结果表明，与DC组相比，给予DWL、DWH和MET的糖尿病大鼠肝AST、ALT、白蛋白、SOD、CAT、GSH和GPX水平显著升高（p < 0.05），与NC组相比无显著差异（p > 0.05）。此外，与DC组相比，给予DWL、DWH和MET的糖尿病大鼠GGT和MDA水平以及DNA片段化和蛋白质羰基水平显著降低（p < 0.05），与NC组相比无显著差异（p > 0.05）。此外，组织学检查显示，给予DWL、DWH和MET的糖尿病大鼠肝细胞恢复正常。因此，可以推断，威尔威茨氏黄檀叶中富含生物碱的提取物可能有助于改善糖尿病大鼠的肝脏损伤。
