Jia Tianchang, Xu Tingting, Xia Jitao, Liu Shuang, Li Wenqiang, Xu Ruidong, Kong Jie, Zhang Qingli
State Key Laboratory of Mariculture Biobreeding and Sustainable Goods; Key Laboratory of Maricultural Organism Disease Control, Ministry of Agriculture; Qingdao Key Laboratory of Mariculture Epidemiology and Biosecurity; Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, Shandong 266071, China; Laboratory for Marine Fisheries Science and Food Production Processes, Laoshan Laboratory, Qingdao, Shandong 266237, China.
State Key Laboratory of Mariculture Biobreeding and Sustainable Goods; Key Laboratory of Maricultural Organism Disease Control, Ministry of Agriculture; Qingdao Key Laboratory of Mariculture Epidemiology and Biosecurity; Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, Shandong 266071, China.
J Invertebr Pathol. 2023 Nov;201:108002. doi: 10.1016/j.jip.2023.108002. Epub 2023 Oct 12.
A new emerging disease called "translucent post-larvae disease" (TPD) in Penaeus vannamei, caused by a novel type of highly lethal Vibro parahaemolyticus (V), has become an urgent threat to the shrimp farming industry in China. In order to develop an effective disinfectant for the prevention and control of the V, the clinical protective effects of polyhexamethylene biguanide hydrochloride (PHMB) against V in Penaeus vannamei were investigated by carrying out an acute toxicity test of PHMB on post-larvae of P. vannamei and its effect of treatment test on V infection. The results showed that the median lethal concentration of disinfectant (LC) values of PHMB to post-larvae of P. vannamei after treatment for 24 h, 48 h, 72 h, 96 h were 16.13 mg/L (14.18-18.57), 10.77 mg/L (9.93-11.72), 9.68 mg/L (8.53-11.64), 9.14 mg/L (7.70-10.99), respectively. In addition, a clinical trial showed that 1 mg/L PHMB showed a strong protective effect on the post-larvae of shrimp challenged with 10-10 CFU/ml of V. The relative percentage survival (RPS) of 1 mg/L PHMB on post-larvae of P. vannamei challenged with V at 10, 10, 10 and 10 CFU/ml were 63.65 %±6.81, 62.96 %±5.56, 60.00 %±3.75 and 66.67 %±3.75 at 96 hours post infection. The results highlight the clinical protective effects of the PHMB and therefor PHMB can be used as a preventive measure to control early TPD infection in shrimp culture. This study also provides valuable information for the prevention of other bacterial diseases in shrimp culture.
一种名为“半透明幼体后期疾病”（TPD）的新出现疾病，由一种新型高致死性副溶血性弧菌（V）引起，已成为中国对虾养殖业的紧迫威胁。为开发一种有效消毒剂用于预防和控制V，通过对凡纳滨对虾幼体进行聚六亚甲基双胍盐酸盐（PHMB）急性毒性试验及其对V感染的治疗试验，研究了PHMB对凡纳滨对虾中V的临床保护作用。结果表明，PHMB处理24小时、48小时、72小时、96小时后对凡纳滨对虾幼体的消毒剂半数致死浓度（LC）值分别为16.13毫克/升（14.18 - 18.57）、10.77毫克/升（9.93 - 11.72）、9.68毫克/升（8.53 - 11.64）、9.14毫克/升（7.70 - 10.99）。此外，一项临床试验表明，1毫克/升PHMB对受到10 - 10 CFU/毫升V挑战的对虾幼体显示出强大的保护作用。1毫克/升PHMB对受到10、10、10和10 CFU/毫升V挑战的凡纳滨对虾幼体在感染后96小时的相对存活率（RPS）分别为63.65%±6.81、62.96%±5.56、60.00%±3.75和66.67%±3.75。结果突出了PHMB的临床保护作用，因此PHMB可作为预防措施用于控制对虾养殖中早期TPD感染。本研究也为预防对虾养殖中的其他细菌疾病提供了有价值的信息。
