Unidad de Epidemiología y Medicina Preventiva, IUSA, Facultad de Veterinaria, Universidad de Las Palmas de Gran Canaria, C/Trasmontaña s/n, Arucas, 35413, Canary Islands, Spain.
Unidad de Epidemiología y Medicina Preventiva, IUSA, Facultad de Veterinaria, Universidad de Las Palmas de Gran Canaria, C/Trasmontaña s/n, Arucas, 35413, Canary Islands, Spain.
Syst Appl Microbiol. 2023 Nov;46(6):126472. doi: 10.1016/j.syapm.2023.126472. Epub 2023 Oct 7.
In the search for mollicutes in wild birds, six Mycoplasma strains were isolated from tracheal swabs taken from four different species of seabirds. Four strains originated from three Yellow-legged gulls (Larus michahellis) and a Cory's shearwater (Calonectris borealis) from Spain, one from a South African Kelp gull (Larus dominicanus), and one from an Italian Black-headed gull (Chroicocephalus ridibundus). These Mycoplasma strains presented 99 % 16S rRNA gene sequence similarity values with Mycoplasma (M.) gallisepticum. Phylogenetic analyses of marker genes (16S rRNA gene and rpoB) confirmed the close relationship of the strains to M. gallisepticum and M. tullyi. The seabirds' strains grew well in modified Hayflick medium, and colonies showed typical fried egg morphology. They produced acid from glucose and mannose but did not hydrolyze arginine or urea. Transmission electron microscopy revealed a cell morphology characteristic of mycoplasmas, presenting spherical to flask-shaped cells with an attachment organelle. Gliding motility was also observed. Furthermore, serological tests, MALDI-ToF mass spectrometry and genomic studies demonstrated that the strains were different to any known Mycoplasma species, for which the name Mycoplasma bradburyae sp. nov. is proposed; the type strain is T158 (DSM 110708 = NCTC 14398).
在对野生鸟类中的柔膜体纲进行研究时，从 4 种不同海鸟的气管拭子中分离出 6 株支原体。其中 4 株来自西班牙的 3 只黄腿鸥（Larus michahellis）和 1 只冠毛海雀（Calonectris borealis），1 株来自南非的黑背鸥（Larus dominicanus），1 株来自意大利的黑头鸥（Chroicocephalus ridibundus）。这些支原体菌株的 16S rRNA 基因序列相似度均为 99%，与鸡毒支原体（Mycoplasma gallisepticum）最为接近。基于标记基因（16S rRNA 基因和 rpoB）的系统发育分析进一步证实了这些菌株与鸡毒支原体和滑液支原体（Mycoplasma tullyi）的密切关系。这些海鸟源菌株在改良的 Hayflick 培养基中生长良好，其菌落呈典型的煎蛋样外观。它们能够代谢葡萄糖和甘露糖产酸，但不能水解精氨酸或尿素。透射电子显微镜显示了与支原体一致的细胞形态，呈球形至烧瓶形，具有附着细胞器。还观察到了滑动运动。此外，血清学试验、基质辅助激光解吸电离飞行时间质谱（MALDI-ToF MS）和基因组研究表明，这些菌株与任何已知的支原体物种均不同，因此将其命名为柔膜体纲未命名种 bradburyae，其模式菌株为 T158（DSM 110708=NCTC 14398）。
