Dental Materials Science, Division of Applied Oral Sciences and Community Dental Care, Faculty of Dentistry, The University of Hong Kong, Hong Kong SAR, P. R. China.
Paediatric Dentistry, Faculty of Dentistry, The University of Hong Kong, Hong Kong SAR, P. R. China.
Dent Mater. 2023 Dec;39(12):1105-1112. doi: 10.1016/j.dental.2023.10.004. Epub 2023 Oct 13.
The aim of present study was to examine the effect of Porphyromonas gingivalis (P.g.) adhesion on dental zirconia by characterizing the physical and chemical properties.
Eighty polished-sintered zirconia discs were prepared and randomly distributed to 5 groups (n = 16): Zirconia cultured with - Group 1: broth containing P.g. for - 3 days; Group 2: 7 days; Group 3: broth (alone) for - 3 days; Group 4: 7 days; and Group 5: dry discs (negative control). After experimental period, broths were analyzed for pH and Zr release with inductively coupled plasma-optical emission spectroscopy (ICP-OES). The zirconia surface was evaluated by scanning electron microscope (SEM), atomic force microscopy (AFM), X-ray diffraction (XRD), water contact angle (WCA), and biaxial flexural strength (BFS).
The mean pH with zirconia adhesion to P.g. group was significantly higher than the broth control (p < 0.05). As per ICP-OES, Zr ion/particulate release with P.g. adhesion to zirconia were significantly higher than the controls (p < 0.05). Post-experimental incubation, no defects were found on zirconia surfaces; tetragonal phase remained constant with no transformation to monoclinic phase but lower peak intensities were identified in experimental groups. WCA of zirconia surfaces with P.g. bacteria for 3 days (12.04° ± 2.05°) and 7 days (15.09° ± 2.95°) were significantly higher than zirconia surfaces immersed with broth (only) for 3 days (7.17° ± 1.09°) and 7 days (7.55° ± 0.65°), respectively (p < 0.05). BFS values of zirconia with P.g. for 3 days (632.57 ± 119.96 MPa) and 7 days (656.17 ± 100.29 MPa) were significantly lower than zirconia incubated in broth alone (765.01 ± 20.12 MPa) conditions (p < 0.05).
Under the conditions of present study, it can be concluded that P.g. adhesion on zirconia leads to structural alterations of dental zirconia further contributing to zirconia degradation.
本研究旨在通过研究物理和化学特性来检测牙龈卟啉单胞菌（P.g.）对牙科氧化锆的黏附作用。
制备 80 个抛光烧结氧化锆片，并将其随机分为 5 组（n=16）：- 组 1：含 P.g.的肉汤培养 3 天；- 组 2：7 天；- 组 3：肉汤（单独）培养 3 天；- 组 4：7 天；- 组 5：干燥片（阴性对照）。实验期结束后，用等离子体发射光谱法（ICP-OES）分析肉汤的 pH 值和 Zr 释放量。用扫描电子显微镜（SEM）、原子力显微镜（AFM）、X 射线衍射（XRD）、水接触角（WCA）和双轴弯曲强度（BFS）评估氧化锆表面。
与肉汤对照组相比，有 P.g.黏附的氧化锆组的平均 pH 值显著更高（p<0.05）。根据 ICP-OES，与对照组相比，有 P.g.黏附的氧化锆中 Zr 离子/颗粒的释放量显著更高（p<0.05）。实验孵育后，氧化锆表面无缺陷；四方相保持不变，没有向单斜相转变，但实验组的峰值强度较低。有 P.g.细菌培养 3 天（12.04°±2.05°）和 7 天（15.09°±2.95°）的氧化锆表面的 WCA 明显高于仅用肉汤培养 3 天（7.17°±1.09°）和 7 天（7.55°±0.65°）的氧化锆表面（p<0.05）。有 P.g.培养 3 天（632.57±119.96 MPa）和 7 天（656.17±100.29 MPa）的氧化锆的 BFS 值明显低于单独用肉汤培养的氧化锆（765.01±20.12 MPa）（p<0.05）。
在本研究条件下，可以得出结论，P.g.在氧化锆上的黏附作用导致牙用氧化锆的结构发生改变，进一步导致氧化锆降解。
