Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences/The Key Laboratory of Biology and Genetic Improvement of Oil Crops, The Ministry of Agriculture and Rural Affairs, Wuhan 430062, China.
Plant Dis. 2024 Apr;108(4):847-851. doi: 10.1094/PDIS-08-23-1571-SC. Epub 2024 Apr 1.
Clubroot disease caused by the soil-borne is devastating to Brassicaceae crops and spreading rapidly in China in recent years, resulting in great yield losses annually. Virulence of populations specializes and is in dynamic change in the fields. Information on the pathotypes and their distributions is crucial to control the clubroot disease. Presently, the pathotypes of prevalent in China, however, are not well determined. In this study, we used 16 hosts, including the European Clubroot Differential (ECD) and Williams sets, to designate the pathotypes of 33 populations from 13 provinces. The 33 populations could be divided into 26 pathotypes by the ECD set or seven pathotypes by the Williams set, revealing ECD16/15/31 and ECD16/31/31 or P4 and P2 as the predominant pathotypes. We found that the differentials ECD01 to ECD04 showed stable and high levels of resistance to most pathotypes of in China, thereby providing valuable resources for clubroot-resistance breeding of Brassicaceae crops. The ECD set exhibited much higher discernibility and further divided the isolates that belonged to the P4 pathotype into 10 ECD pathotypes. Isolates of ECD16/23/31 and ECD16/15/31 were strongly virulent on Huashuang 5R, the first and widely used clubroot-resistant cultivar of oilseed rape in China. As we learn, 26 pathotypes are the most diverse populations of characterized until now in China. Our study provides new insights into virulence specialization of and their geographical distributions, contributing to exploitation of clubroot-resistant resources and the field layout of the present resistant crops in China.
根肿病是一种由土壤传播的疾病，对十字花科作物造成严重破坏，近年来在中国迅速蔓延，每年造成巨大的产量损失。在田间，种群的毒力具有特异性并处于动态变化中。关于致病型及其分布的信息对于控制根肿病至关重要。然而，目前在中国流行的致病型尚未得到很好的确定。在本研究中，我们使用了 16 种寄主，包括欧洲根肿病鉴别寄主（ECD）和威廉姆斯鉴别寄主，对来自 13 个省份的 33 个种群的致病型进行了鉴定。33 个种群可根据 ECD 集分为 26 个致病型，或根据威廉姆斯集分为 7 个致病型，表明 ECD16/15/31 和 ECD16/31/31 或 P4 和 P2 是主要的致病型。我们发现，不同的鉴别寄主 ECD01 至 ECD04 对中国大多数 致病型表现出稳定和高水平的抗性，从而为十字花科作物的根肿病抗性育种提供了有价值的资源。ECD 集表现出更高的辨别力，并进一步将属于 P4 致病型的分离物分为 10 个 ECD 致病型。ECD16/23/31 和 ECD16/15/31 的分离物对中国第一个也是广泛使用的油菜根肿病抗源品种华双 5R 具有很强的毒性。据我们所知，到目前为止，这是在中国鉴定的 最具多样性的 种群。我们的研究为 毒力特化及其地理分布提供了新的见解，有助于开发根肿病抗性资源和中国现有抗性作物的田间布局。
